Question

Before lysis we added lysozyme to weaken the bacterial cell walls. back in chapter 6 we studied this enzyme as part of the lecture portion of the class. what was interesting about hen egg white lysozyme?

a) it is unclear if it uses an SN1 or SN2 type mechanism, and the debate continues to this day

b) it is only active below pH 7.2 because of a protonated histidine residue

c) it was the first enzyme to have its primary sequence determined

d) it uses an interesting carbanion intermediate

Answer 1

The other options are incorrect, its isoelectric point is around 11 and it doesn't even have a His residue.

d) it uses an interesting carbanion intermediate