A sodium phosphate buffer has a concentration of 25 mM. If you take 25 mL of the buffer and add 75 mL of water, what is the final concentration after the dilution? Show work.
A sodium phosphate buffer has a concentration of 25 mM. If you take 25 mL of...
If you add 2 mL of 1M HCl to 600 mL of 25 mM phosphate buffer (pH 8.0), what will be the resulting pH? (pKa of phosphate = 7.21)
Sample Buffering Capacity Problem Suppose you have 600 ml of a sodium phosphate buffer where Concentration = .25M Ratio of Acid : Base = 2:3 PK, of Sodium dihydrogen phosphate = 7.21 Next we add 150 ml of .2M HCL How much does the pH change after this addition?
In a solution of 250 mM sodium phosphate buffer (so the concentration represents all forms of phosphate no matter the ionization state) at pH 5.2 (the pH of some synaptic vessicles), what is the ratio of H2PO4- to HPO4-2 ? The pKa's of phosporic acid are 2.15, 6.86 and 12.32. Set up the equation to solve for the answer (you aren't expected to give a numerical answer). Is this an appropriate buffer at this pH? Why or why not?
A student was told to make a pH = 7.5 buffer from sodium dihydrogen phosphate and sodium hydrogen phosphate with an ionic strength of 0.10 a) What concentration of Na2HPO4 and NaH2PO4 need to be used? b) If 10.0 mL of 1.00 M Hbr is added to 1.00 L of this buffer, what is the pH? Please show work.
3. How would you make 200 ml of a 250 mM phosphate buffer from a 1M stock solution of phosphate buffer? r. nus: how would you make 100 ul of 10 mM glucose solution from a 1M glucose stock solution? int: might need to do a serial dilution, as our pipettes cannot dispense less than 2ul)
please hlep me answer those three questions asap. Please ignore question 7. Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Calculate the sodium acetate molar concentration of each dilution. Record the calculated values in Data Table 2. The concentration of the 4.7 pH buffer is 0.05 M sodium acetate. Concentration(start) x Volume(start) = Concentration(final) x Volume(final) pH 4.7 buffer (10 mL sodium acetate, 10 mL water) 1st dilution (5 mL pH 4.7 buffer solution, 15 mL water) 2nd dilution (5mL of 1st dilution, 15mL water) 3rd dilution (5mL of 2nd dilution, 15mL water) Calculation Help 5mL if 0.1M sodium acetate...
Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. The buffer helps to maintain a constant pH. The osmolality and ion concentrations of the solution usually match those of the human body. A) You need to prepare a stock solution at pH 7.00 with KH2PO4 and Na2HPO4 (pKa =7.21). What would be the respective concentration of these substances if you wish to obtain the final phosphate concentration [HPO4 −2 ] + [H2PO4 − ] = 0.3...
You are given 75 mL of 0.70 M acetic acid/acetate buffer to test. The starting composition of the two major species are: Concentration of CH,COOH: 0.250 M Concentration of CH,COO: 0.450 M a. Calculate the initial pH of the buffer. Clearly show all work required to arrive at your answer. b. You add 1.0 mL of 2.00 M HCl to the buffer. Calculate the molarity of H, O' added as HCl, and the final molarities of acetic acid and acetate...
When you shake a sample of 1mL phycocyanin and 5 mL sodium phosphate buffer, there may be frothing. What would cause this?