When you shake a sample of 1mL phycocyanin and 5 mL sodium phosphate buffer, there may...
When you shake a sample of 1mL phycocyanin and 5 mL sodium phosphate buffer, there may be frothing. What would cause this?
Homework Answers
As protein are amphiphilic and act as surfactants . They can adsorb at air -water interface and lower the surface tension of the solution which allows the formation of foam . Shaking induce mechanical denaturation of proteins. These denaturated protein displays surfactant properties and they give rise to frothing in aqueous solution .
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When you shake a sample of 1mL phycocyanin and 5 mL
sodium phosphate buffer, there may...
Sample Buffering Capacity Problem Suppose you have 600 ml of a sodium phosphate buffer where Concentration...
Sample Buffering Capacity Problem Suppose you have 600 ml of a sodium phosphate buffer where Concentration = .25M Ratio of Acid : Base = 2:3 PK, of Sodium dihydrogen phosphate = 7.21 Next we add 150 ml of .2M HCL How much does the pH change after this addition?
If 5 mL of 0.1M HCl is added to 50 mL of 0.1M sodium phosphate buffer...
If 5 mL of 0.1M HCl is added to 50 mL of 0.1M sodium phosphate buffer at pH 7.0, what is the resulting pH?
A sodium phosphate buffer has a concentration of 25 mM. If you take 25 mL of...
A sodium phosphate buffer has a concentration of 25 mM. If you take 25 mL of the buffer and add 75 mL of water, what is the final concentration after the dilution? Show work.
What masses of monobasic and dibasic sodium phosphate will you use to make 250 mL of...
What masses of monobasic and dibasic sodium phosphate will you use to make 250 mL of 0.1 M sodium phosphate buffer, pH = 7?
1) How would you make 300 ml of a 0.1 M sodium phosphate buffer, pH 7.0...
1) How would you make 300 ml of a 0.1 M sodium phosphate buffer, pH 7.0 (pKa = 7.2) phosphate dibasic (the conjugate base). 2) What are the concentrations of acetate and acetic acid in a 0.2 M acetate buffer pH 5.3? The pKa for acetic acid is 4.76. 3)You have 100 ml of 0.1 M acetate buffer pH 5.2 (pKa 4.76). You add 10 ml of 0.1 M NaOH. Calculate the resulting change in pH and the buffering capacity...
To prepare a buffer of 7.00, a 2.00 M solution of sodium dihydrogen phosphate is prepared....
To prepare a buffer of 7.00, a 2.00 M solution of sodium dihydrogen phosphate is prepared. To make the buffer, sodium hydrogen phosphate is to be added. What mass of sodium hydrogen phosphate should be measured out to make 500 ml of the buffer?
How you would prepare a solution of 250.0 mL of a 0.150M phosphate buffer at ph=7....
How you would prepare a solution of 250.0 mL of a 0.150M phosphate buffer at ph=7. (phosphoric acid pka1=2.12, pka2=7.21, pka3=12.32). What would the pH of the buffer be if 5.00mL of 1.10M HCL was added to 100mL sample of the prepared buffer.
You are instrucbed to create 900. mL of a 0.59 M phosphate buffer with a pH...
You are instrucbed to create 900. mL of a 0.59 M phosphate buffer with a pH of 6.4. You have phosphoric acid and the sodium salts NaH2PO4, Na2HPO4, and Na3PO4 available. numerical answers to three significant H3PO4(s)H20)H3 aH2PO4(aq) H2PO4-(aq) + H2O(りーH3O + (aq) + HP042-(aq) Ka2-6.2×10-8 HPO()H20()H3)PO43 (aq) Ka 4.8x10-13 Ka 6.9x10-2 Which of the available chemicals will you use for the acid component of your buffer? O H3PO4 O Na2HPO Na3PO4 Which of the available chemicals will you use...
A student was told to make a pH = 7.5 buffer from sodium dihydrogen phosphate and...
A student was told to make a pH = 7.5 buffer from sodium dihydrogen phosphate and sodium hydrogen phosphate with an ionic strength of 0.10 a) What concentration of Na2HPO4 and NaH2PO4 need to be used? b) If 10.0 mL of 1.00 M Hbr is added to 1.00 L of this buffer, what is the pH? Please show work.
Using a 0.25 M phosphate buffer with a pH of 7.0, you add 0.79 mL of...
Using a 0.25 M phosphate buffer with a pH of 7.0, you add 0.79 mL of 0.49 M HCl to 42 mL of the buffer. What is the new pH of the solution? Using a 0.25 M phosphate buffer with a pH of 7.0, you add 0.79 mL of 0.49 M NaOH to 42 mL of the buffer. What is the new pH of the solution?
Sample Buffering Capacity Problem Suppose you have 600 ml of a sodium phosphate buffer where Concentration = .25M Ratio of Acid : Base = 2:3 PK, of Sodium dihydrogen phosphate = 7.21 Next we add 150 ml of .2M HCL How much does the pH change after this addition?
You are instrucbed to create 900. mL of a 0.59 M phosphate buffer with a pH of 6.4. You have phosphoric acid and the sodium salts NaH2PO4, Na2HPO4, and Na3PO4 available. numerical answers to three significant H3PO4(s)H20)H3 aH2PO4(aq) H2PO4-(aq) + H2O(りーH3O + (aq) + HP042-(aq) Ka2-6.2×10-8 HPO()H20()H3)PO43 (aq) Ka 4.8x10-13 Ka 6.9x10-2 Which of the available chemicals will you use for the acid component of your buffer? O H3PO4 O Na2HPO Na3PO4 Which of the available chemicals will you use...
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