The process of DNA replication yields two identical DNA molecules from a single double-stranded molecule. Cellular proof-reading and error-checking mechanisms ensure nearly perfect fidelity of the DNA copies. However, RNA viruses lack replication error-checking mechanisms, and thus have higher rates of genetic variation. Discuss the importance of error-checking mechanisms during replication and how in the absence of these mechanisms genetic variation is introduced.
Importance of error-checking mechanisms during replication: error-checking mechanism of replication that detects and remove copy errors.base selection , proof reading and mis match repair. it is a rare for copyerror to pass through all three stages without being corrected. the neat geometry of dna helix play an important role in detection of errors. correct pairs A with T G with C fit snugly in the dna helix.in correct pairs do not.distortions to the helix caused by damage or mispairing under-lies each of the three error checking stages of replication, in the absenece of error checking mechanism genetic variation is introduced through mutation.
The process of DNA replication yields two identical DNA molecules from a single double-stranded molecule. Cellular...
The process of DNA replication yields two identical DNA molecules from a single double-stranded molecule. Cellular proof-reading and error-checking mechanisms ensure nearly perfect fidelity of the DNA copies. However, RNA viruses lack replication error-checking mechanisms, and thus have higher rates of genetic variation. Discuss the importance of error-checking mechanisms during replication and how in the absence of these mechanisms genetic variation is introduced.
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...