in setting up their dot blot experiment, john and sarah forgot to boil the DNA samples...
in setting up their dot blot experiment, john and sarah forgot to boil the DNA samples at the very beginning but everything else was done correctly. what do you think will be outcome of the experiment (success or failure) and why?
Homework Answers
The dot blot technique is an experimental technique in molecular biology and is used to detect the proteins. In this technique it is very important to heat or boil the DNA before the method because this helps in denaturing the DNA which is very important for the process and for characterization. In case the DNA sample is not boiled, it will give negative result as the sample has not been denatured.
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in setting up their dot blot experiment, john and sarah forgot
to boil the DNA samples...
You find yourself setting up another Western blot, however. today you are preparing the samples t...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
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You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
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Please answer the below genetics question and show/explain all
work.
4. You've recently discovered the sequences for two alleles for the mouse gene XCD, which you have creatively named XCD and XCD2. This gene is believed to be involved in fat metabolism, somehow, but you haven't successfully been able to isolate the protein yet. You've also noticed that your mutant grey coat mice tend to be much leaner and muscular than those with the standard white coat. So for your...
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