Question

A series of proteins with known molecular mass and an enzyme of unknown molecular mass are separated by chromatography on a Sephadex G-200 column (range: 5-600 kDa). The elution volume (Ve) for each protein is indicated in the table below. Estimate the molecular mass of the unknown protein.

Protein	MW	Ve (mL)
Blue dextran	2000 kDa	85.00
lysozyme	14 kDa	200.00
Chymotrypsinogen	25 kDa	190.00
ovalbumin	45 kDa	170.00
Serum albumin	65 kDa	150.00
aldolase	150 kDa	125.00
urease	500 kDa	90.00
ferritin	700 kDa	92.00
ovomucoid	28 kDa	160.00
unknown	?	130.00

Thank you!

Answer 1

14	200
25	190
28	160
45	170
65	150
150	125
500	90
700	92
2000	85

A Caliberation plot was plotted and the linear line was obtained by linear regression plot was done and the weight of unknown was found to be 600Kda