what happens to the Phenol Red broth when acids is produced? explain why
Phenol red broth is used to differentiate gram negative enteric bacteria. Phenol red broth contains peptone , phenol red ( a pH indicator) , a durham tube, and one carbohydrate and phenol red is a pH indicator which turns Yellow below the pH of 6.8 and fuchsia above pH of 7.4 and hence since acid( pH of acidic medium is less than 7) is produced then the phenol red broth will change the colour of medium to yellow.
what happens to the Phenol Red broth when acids is produced? explain why
I have a mixture of E. coli, L. Broth, ampicillin, and phenol red. I want to measure the concentration of E. coliusing a spectrophotometer. What do I blank the spec with? (Select all that apply.) A) Ampicillin B) E. coli C) Ethanol D) L. Broth E) NaCl ions F) Penicillin G) phenol red H) Water Also please include just a short explanation, thank you!!
You will be inoculating the Phenol Red tests from a master inoculum of LB broth and your unknown bacteria using a 1:100 dilution. The final volume of the Phenol Red test (with sugar added) is 10 ml. How much master inoculum will you add in mL and uL?
Microbiology Q1 Q2 Q3 A phenol red glucose broth with a Durham tube has been inoculated and incubated. After 48 hours, the broth is turbid and bright yellow and approximately half of the contents of the Durham tube contains bubbles. Interpret these results. The organism will ferment lactose as well. The organism is fermenting glucose. The organism is an obligate anaerobe. The organism is fermenting lactose and producing ethanol and CO2. What can be detected using SIM medium? Check all...
phenol red broth 5-3 5-3 Enter your results color and bubble/no include the relevant enzyme's name in your Phenol Red Broth OBSERVATIONS AND INTERPRETATIONS color and bubble/no bubble in the table below. using the symbols shown in Table 5-2, page 305. Be sure to vant enzyme's name in your interpretation is an organism ferments sucrose and/or lactose Organism PR Base Results PR PR Glucose Lactose PR Sucrose Interpretation Uninoculated control LAC GLU E. Colin LAC SUC GLU Proteus vulgaris LAC...
When performing a presumptive test with a water sample, you typically use a lactose broth with a durham tube and look for the indication of gas as a sign of fermentation. If we use a phenol red lactose broth instead, do we still need to use the durham tube to see if fermentation happened and to determine if th bacteria are nonpotable?
A positive PDase test will result in what color change in the media when the appropriate reagent is added? If fermentation occurs in the phenol-red carbohydrate fermentation broth the media will turn what color?
State what happens to most of the absorbed amino acids when a high-protein meal is ingested
dyes, such as phenol red or methylene blue used in differential media? Why are dyes, such as A. to inhibit motility B. to stain microbes C to kill bacteria D. to see microbes to detect pH changes due to metabolism What is the purpose of differential medias to encourage growth of the desired bacteria B to isolate few bacteria to observe metabolism of the bacteria D. to stain bacteria E to determine motility 10 Why does Staphylococcus aureus turn yellow...
1. Why is it important to mix the phenol with sodium hydroxide before adding the chlorobutane? What happens during this time? 2. Why does phenol react with sodium hydroxide? Why does ethanol not react to the extent that phenol does with sodium hydroxide? 3. What is the better nucleophile, sodium phenoxide or phenol? Why? 4. Why do you need to rinse your product with ice water instead of room temperature water?
answer all... please (1 pts) 16) Indicate whether phenol red lactose fermentation broth is a selective or differential media by marking the appropriate box(es). If the medium is both selective and differential, mark both. If the medium is neither selective nor differential, check the "neither" box: selective o differential Oneither (1 pts) 17) Indicate whether mannitol salt agar is a selective or differential media by marking the appropriate box(es). If the medium is both selective and differential, mark both. If...