Question

1. A prokaryotic (bacterial) cell, which is approximately cylindrical in shape cellhas a diameter of 0.35 μm and length of 0.9 μm. An approximately sphericaleukaryotic cell has a radius of 20 μm.

a) Calculate the surface area of each cell type of cell in μm2.

b) Calculate the volume of each cell type in μm3.

c) Compare the surface area to volume ratio of each cell. Given that theconcentration of a glucose transporters is the same in each cell typeand both cells are placed in a test tube containing a solution ofglucose, which cell will be able to increase its cytosolic glucoseconcentration faster?

d) Using unit conversion, transform volume of the prokaryotic cell fromμm3 to mL. (You can assume that 1mL of water is 1 cm3)

e) Given the total mass of protein in a bacterial cell is 2 x 10-14g and theaverage molecular weight of proteins in the cell is 50 kDa (50,000g/mole). Compute the average molar concentration of protein in aprokaryotic cell.

2) If2000moleculesofproteinAwaspresentinaprokaryoticcellwhatwouldthe micromolar concentration of this protein be in the cytosol (Note:Avagadro’s number = 6.022 x 1023molecules/mole)

Answer 1