Bioinformatics. Using the NCBI ORF Finder Web form, determine the open reading frames for the entire...
Bioinformatics.
Using the NCBI ORF Finder Web form, determine the open reading frames for the entire DNA record for accession number EF595246 (coding region plus flank). Based on what you see, how many ORFs do you see? describe your choice of the correct reading frame and why you made that choice. Discuss your other strong choice.
Homework Answers
NCBI ORF finder has identified, a total of 12 ORF in the DNA sequence recorded under the accession number EF595246.
The ORF 4 (+ 3 frame) is selected as correct reading frame.
The ORFs were inspected using SmartBLAST programme against NCBI's nr-database.
The ORF 4 is a longest ORF demonstrating significant similarity with the major ampullate spidroin 1 from Latrodectus hesperus. The query cover was 100% as well as the similarity was 100 %.
The ORF identified by ORF finder can be screened by check their similarity with proteins from different database. Here ORF 4 depicted the significant match as mentioned above.
The other ORFs identifies were
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Bioinformatics.
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