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9.18 In corn, a colored aleurone is due to the presence of an R allele; r/r...

9.18 In corn, a colored aleurone is due to the presence of an R allele; r/r is colorless. Another gene controls the color of the plant, with g/g being yellow and G_ being green. A plant of unknown genotype is test-crossed, and the following progeny plants were obtained.

Colored green 89

Colored yellow 13

Colorless green 9

Colorless yellow 92

  1. What was the phenotype and genotype of the plant used for the test cross?

  2. What were the phenotypes of the plants used to produce the plant with the unknown genotype? (Assume that the parentals were homozygous.)

  3. What is the approximate map distance between the R locus and the G locus?

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Answer #1

here the progenies of testcross include all four phenotypes that is

Colored green

Colored yellow

Colorless green

Colorless yellow

so the genotype of the plant crossed to the tester (a tester is homozygous recessive ) has all four alleles, which means the plant is dihybrid.

RrGg * rrgg

RG Rg rG rg
rg RrGg (colored green) Rrgg ( colored, yellow) rrGg (colorless, green) rrgg (colorless, yellow)

if the genes are not linked all four phenotypes are expected in the equal proportion that is 25% each but here all four phenotypes are not observed in equal proportion.

Colored green 89

Colored yellow 13

Colorless green 9

Colorless yellow 92

the recombination frequency in percentage is the distance between genes in the map units, maximum recombination ( when the genes are not linked ) is 50%, so if the genes are linked the recombination frequency is less than 50%.

recombination frequency= ( number of recombinant progenies/total number of progenies)100

so recombination frequency is the percentage of recombinant progenies if the genes are linked the number of recombinant progenies is less than the number of parental type progenies, so here

Colored yellow 13

Colorless green 9

are recombinant progenies, Rrgg ( colored, yellow) and rrGg (colorless, green) are recombinant progenies developed from the recombinant gametes Rg and rG respectively so the parental type gametes are RG and rg.

so RG alleles came from one parent and rg came from another parent so the genotype of the true-breeding parents which gave rise to dihybrid is RRGG and rrgg.

recombination frequency= ( (number of colored yellow+ number of colorless green )/ total number)100

= ( ( 13+9)/203)100

= 10.84 %

genotype of the plant is RG/rg

genotype of the parents are RRGG and rrgg

distance between the genes is 10.84 cM.

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