I weighed out 0.0036 g of estradiol (M.W. 272.4 g/mol) in a dry vial. How much ethanol must I dissolve the steroid in to yield a 10 mM solution?
Ans :
Number of mol of estradiol = given mass / molar mass
= 0.0036 g / 272.4 g.mol-1
= 1.32 x 10-5 mol
Molarity = 10mM = 1.0 x 10-4 M
M = number of mol of solute / volume of solution in L
1.0 x 10-4 = 1.32 x 10-5 / V
V = 0.132 L
or 132 mL
So it must be dissolved into 132 mL of ethanol.
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