Questions: 1)why was the membrane incubated in blocking buffer? What would be the consequences of failing...
Questions:
1)why was the membrane incubated in blocking buffer? What would be the consequences of failing to block the membrane?
2) The molecular mass of Myosin Light Chain is approximately 22 KD, myosin heavy chain is 210 KD and actin is 42KD. Which protein will migrate the fastest through the gel and why?
Homework Answers
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Questions:
1)why was the membrane incubated in blocking buffer? What would
be the consequences of failing...
1) Why was the membrane incubated in blocking buffer? What would be the consequence of failing...
1) Why was the membrane incubated in blocking buffer? What would be the consequence of failing to block the membrane? 2) Explain the process of loading protein ladder and actin/myosin protein onto the gel with the fish samples and how it is necessary for analysis of the gel and immunoblot? 3) Why was a Bradford assay performed with the fish protein extracts? Fully explain why this was necessary for analysis of the gel and immunoblot? 4) The molecular mass of...
In the Western blot experiment, )Why are proteins treated with ionic detergent (SDS), reducing agents (DTT), and heat before SDS-PAGE Why do SDS-coated proteins migrate in an electric field? e...
In the Western blot experiment, )Why are proteins treated with ionic detergent (SDS), reducing agents (DTT), and heat before SDS-PAGE Why do SDS-coated proteins migrate in an electric field? e molecular mass of myosin light chain I is approximately 22 kD, myosin heavy chain is 200 kD ane actin is 42 kD, Which proteins will migrate fastest through the gel? Why?
In the Western blot experiment, )Why are proteins treated with ionic detergent (SDS), reducing agents (DTT), and heat before...
1) Explain the process of loading protein ladder and actin/myosin protein onto the gel with the...
1) Explain the process of loading protein ladder and actin/myosin protein onto the gel with the fish samples and how it is necessary for analysis of the gel and immunoblot? 2) Why was the Bradford assay performed with the fish protein extracts? Fully explain why this was necessary for analysis of the gel and immunoblot. 3) The molecular mass of Myosin Light chain is approximately 22KD, myosin heavy chain is 210KD and actin is 42KD. Which protein will migrate the...
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has...
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has an observance reading of 0.015 at 260 nm? a. 6 ug mL b. 60 ug/mL c. 75 ug/mL d. 750 ug/mL 8- When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of a. 20 6.30 c. 40 d. 50 9-DNA is isolated from a clinical sample. The absorbance at 260...
help with questions 5 to 10 please PCB 3023L Lab #4 Protocol & Worksheet (30pt) You...
help with questions 5 to 10 please
PCB 3023L Lab #4 Protocol & Worksheet (30pt) You may work in your lab groups durine class. but all written answers must be completed individually in your own words. 1) Using the plasmid map for orientation 1 and the cDNA map as a guide, complete the plasmid map for orientation #2. (4pt) 612 1318 1 - EcoRi EcoRI Xbal ECORV -Xbal- 1662 +Bell EcoRI EcoRV Not FP -- Xhol X 2015 PRSP +...
Discussion Questions: 1. What is the purpose of a distribution center? 2. Why does Cameron have...
Discussion Questions: 1. What is the purpose of a distribution center? 2. Why does Cameron have eight warehouses? Why do you think Kelly believes six warehouses is better than eight? 3. What is the cost of holding inventory at Cameron? 4. Why should the decision to invest in inventory be different from investing in capital? 5. Do you think it is prudent to evaluate the future of the Atlanta warehouse independently from consideration of the other seven warehouses in the...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
In the Western blot experiment, )Why are proteins treated with ionic detergent (SDS), reducing agents (DTT), and heat before SDS-PAGE Why do SDS-coated proteins migrate in an electric field? e molecular mass of myosin light chain I is approximately 22 kD, myosin heavy chain is 200 kD ane actin is 42 kD, Which proteins will migrate fastest through the gel? Why?
In the Western blot experiment, )Why are proteins treated with ionic detergent (SDS), reducing agents (DTT), and heat before...
18 15 16 17 7- What is the concentration of DNA whereby a 1:100 dilution has an observance reading of 0.015 at 260 nm? a. 6 ug mL b. 60 ug/mL c. 75 ug/mL d. 750 ug/mL 8- When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and a conversion factor of a. 20 6.30 c. 40 d. 50 9-DNA is isolated from a clinical sample. The absorbance at 260...
help with questions 5 to 10 please
PCB 3023L Lab #4 Protocol & Worksheet (30pt) You may work in your lab groups durine class. but all written answers must be completed individually in your own words. 1) Using the plasmid map for orientation 1 and the cDNA map as a guide, complete the plasmid map for orientation #2. (4pt) 612 1318 1 - EcoRi EcoRI Xbal ECORV -Xbal- 1662 +Bell EcoRI EcoRV Not FP -- Xhol X 2015 PRSP +...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
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