Draw (or describe) a dilution scheme for making the following bacteriophage titer plates: 1:100 (10-2), 1:1000...
Draw (or describe) a dilution scheme for making the following bacteriophage titer plates: 1:100 (10-2), 1:1000 (10-3), and 1:10,000 (10-4) using a maximum of 2 dilution blanks. Show (or include in your description) all of the volumes including transfers, amounts in tubes and amounts plated.
Homework Answers
Dilution of plate = dilution of tube × volume plated
Dilution of tube = initial volume / final volume
Total dilution of tube = multiply previous dilutions
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Draw (or describe) a dilution scheme for making the following
bacteriophage titer plates: 1:100 (10-2), 1:1000...
Suppose you would like to determine the number of bacteria in a culture via an aerobic...
Suppose you would like to determine the number of bacteria in a culture via an aerobic plate count. Explain (with pictures) how you would set up a dilution series (104, 106, 10) using the fewest number of dilution blanks. Draw and label your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated 1. You want to set up a 1:10 dilution series, so that you can plate a 10-1, 102, and a...
3. (2 points) Culture C has a titer of 2.3 x 107 bacteria/mL. Draw a dilution...
3. (2 points) Culture C has a titer of 2.3 x 107 bacteria/mL. Draw a dilution scheme showing 1 method in which this culture could be diluted to give plates with between 30 and 300 colonies.
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial cu...
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
#1) What should be the concentration of kanamycin if a serial 2-fold dilution was done starting...
#1) What should be the concentration of kanamycin if a serial 2-fold dilution was done starting with tube 1 and ending in tube 9 with an initial concentration of 100 ug/mL tube # 1 2 3 4 5 6 7 8 9 10 [control] Kanamycin concentration (ug/ml) 100 ? ? ? ? ? ? ? ? 0 growth no no no no no yes yes yes yes yes #2) Culture C has a titer of 2.3 x 107 bacteria/mL. Draw...
Answer the following questions regarding a 10x Serial Dilution experiment using yeast cell cultures from tube...
Answer the following questions regarding a 10x Serial Dilution experiment using yeast cell cultures from tube A. Tube A contains 1 ml of yeast culture. 3.6 ml of sterile water is placed into 4 tubes (B, C, D, and E). 0.4 ml of yeast culture is removed from tube A and put into tube B.. 0.4 ml is transferred from tube B to tube C, and from tube C to tube E. 1.) What is the final volume in tubes...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
Dilutions. In the following example: a. What is the titer of the virus? b. Is the...
Dilutions. In the following example: a. What is the titer of the virus? b. Is the virus titer high enough of the newly arrived virus shipment to be used as a control in the experiment? c. Show your work Note: The virus titer needed as a control in the experiment was at least 1 x 10^7 viruses per ml. The control virus stock arrived from the ATCC distributor arrived frozen and was labeled as 3x10^8 viruses/ml (equal to 300,000,000 viruses/ml...
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32,...
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32, and 1:64, there is no visible agglutination in the 1:64 tube. What is the titer of the antibody? A. 32 B. 16 C. 64 D. Cannot be determined 2. If you want to make 2 mL of a 1:20 dilution, how much serum would be needed? 3. When 1.0 mL of serum is added to 3.0 mL of diluent, what dilution does this represent?...
Winogradsky Columns A) What is the metabolic process that utilizes oxygen in the first few weeks?...
Winogradsky Columns A) What is the metabolic process that utilizes oxygen in the first few weeks? B) What is the metabolic process that re-introduces oxygen towards the end? C) Cellulose served an important purpose in driving the metabolism of the column. What metabolic pathway did cellulose allow organisms in the column to partake in? Forgetting to add cellulose in the column means that ____________ was not produced, which means that ____________ couldn’t be turned into ___________ by anaerobic bacteria. D)...
For the 1.5" shaft shown below: 10 in 2 B 1 200 lbf 10 in 1000 lbf 500 lbf 100 lbf 1. Draw the FB...
For the 1.5" shaft shown below: 10 in 2 B 1 200 lbf 10 in 1000 lbf 500 lbf 100 lbf 1. Draw the FBD with all corresponding forces (10 points) 2. Draw the shear diagram with all corresponding values (20 points) 3. Draw the moment diagram with all corresponding values (20 points) 4. Find the location with the highest stress, what is the stress at this location? (20 points) 5. Use Mohr's circle or von Misses criteria to find...
Suppose you would like to determine the number of bacteria in a culture via an aerobic plate count. Explain (with pictures) how you would set up a dilution series (104, 106, 10) using the fewest number of dilution blanks. Draw and label your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated 1. You want to set up a 1:10 dilution series, so that you can plate a 10-1, 102, and a...
3. (2 points) Culture C has a titer of 2.3 x 107 bacteria/mL. Draw a dilution scheme showing 1 method in which this culture could be diluted to give plates with between 30 and 300 colonies.
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
b. 1 ml 1ml 「-I Final dilution 4(1 point) This represents a serial dilution to determine viable bacterial counts. Indicate the final dilutions in each of the two tubes. Note that two different volumes of the cells intube #2 are plated onto agar plates A and B. Indicate the dilution factor that will be used to report the original cell concentration on a per mL basis. 1 ml 1 ml Final dilution in Tube #1 = Final dilution in Tube...
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32, and 1:64, there is no visible agglutination in the 1:64 tube. What is the titer of the antibody? A. 32 B. 16 C. 64 D. Cannot be determined 2. If you want to make 2 mL of a 1:20 dilution, how much serum would be needed? 3. When 1.0 mL of serum is added to 3.0 mL of diluent, what dilution does this represent?...
For the 1.5" shaft shown below: 10 in 2 B 1 200 lbf 10 in 1000 lbf 500 lbf 100 lbf 1. Draw the FBD with all corresponding forces (10 points) 2. Draw the shear diagram with all corresponding values (20 points) 3. Draw the moment diagram with all corresponding values (20 points) 4. Find the location with the highest stress, what is the stress at this location? (20 points) 5. Use Mohr's circle or von Misses criteria to find...
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