Homework Answers
|
Stock Concentration is |
2.3 |
10000000 |
(Stock 1) 107 |
1 |
|
Take 100uL from Stock 1 and add in 10mL |
2.3 |
100000 |
(Stock 2) 105 |
1/100 |
|
Take 100uL from Stock 2 and add in 10mL |
2.3 |
1000 |
(Stock 3) 103 |
1/10000 |
|
Take 100uL from Stock 2 and add in 1mL |
2.3 |
100 |
(Stock 4) |
1/100000 |
|
So, Stock 4 is having cells number per mL is |
2300 |
As we have Cell concentration that is 2300 and desired concentration required is 30 and 300 colonies so we need to further dilute the samples.
Since 1mL stock is having 2300 cells so 0.1 mL will have =2300/10
= 230 cells
0.1ml = 230 Cells
Volume required for 300 colonies = (300/230)/10
= 0.130 mL
Similarly for 30 colonies, 2300 in 1 ml, 230 in 0.1 ml and 23 in 0.01mL (1/100 mL)
Volume required for = (30/23)/100
=0.0130 mL
Finally, volume required for 30 and 300 colonies would be 0.0130 mL and 0.130mL from stock 4
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