A microscope objective is used with a numerical aperture NA= 1.0 and immersion oil of refractive index n = 1.414. Assuming that the wavelength of light λ =0.486 µm, find the resolution of the microscope and its aperture angle α.
A microscope objective is used with a numerical aperture NA= 1.0 and immersion oil of refractive...
18. For the following objective lens, if the refractive index η is 1.51 and the half angle of the angular aperture μ is20: what is the numerical aperture of this objective lens? If the light with 400nm is used for this microscopy, maximum resolution of this objective lens? (10 points) what is the Angular Aperture Hone Light Cone- Figure 1 18. For the following objective lens, if the refractive index η is 1.51 and the half angle of the angular...
(1 point) READING 4 The resolution of a microscope is diffraction limited, just as the eye, but gains a significant advantage because an object can be placed very close to its objective lens. The smallest resolvable linear separation xTor an ordinary light microscope is: where λ is he wavelength o light used, and Θ ls he half Viewing angle 0 he obiective The quantity n sne s ca e the num enca aperture of he obiective where n s the...
Light of wavelength 589 nm is used to view an object under a microscope. The objective lens of the microscope is the lens that gathers light from the object being observed and focuses the light rays in order to form an image of the object. It acts as the aperture of the system, determining its resolution. In this case, the objective lens is circular, of diameter 0.95 cm. Assuming that the resolution of the microscope depends solely on the diffractive...
A step index fibre has a numerical aperture of 0.16, a core refractive index of 1.45 and a core diameter of 90 mm. Calculate: 1. The refractive index of the cladding. 2. The acceptance angle θm and the confinement angle θ 0 m. 3. The approximate number of modes with a wavelength λ = 0.9 µm that the fibre can carry.
you are using a microscope with an objective lens with a numerical aperture of 0.75 and light source with a wavelength of 500nm what is the R?
The objective of a microscope has magnifying power of 20×, a numerical aperture of 0.4, a working distance (WD) of 4 mm (~f ). For a wavelength of light of 450 nm, what is the resolving power of this objective? What is the diameter of its lens?
Objective lens Refracted (lost) light rays, VY Immersion oil Slide Condenser - ( Light source Figure 2 Refractive index in air and in mineral oil Carrera attachment Eyeplece focular lens) Light pathway LU Arm Body tube Objective Coarse and fine focusing adjustment knob Stage Light source Spircimen Condenser Illuminator Figure 3 Pathway of light in a compound scope Microscopy/Microscopic Examination of Stained Cell Preparations Q8 Use Figure 2 and 3 in the lab info to answer the following question. Which...
please please help with all of them no thorough explanation needed! 5. Select the most appropriate answer. When using a dichotomous key outside to identify unknown organisms one should A. not remove parts of the plant or disrupt the ecosystem B. be aware of hazards such as ticks and poison ivy C. should write down the steps followed on the key. D. Be aware that your best attempts to identify a specimen might be disrupted by the presence of sexual...
2. A microscope has a 1.1 cm diameter objective lens with a focal length of 2.3 mm. The microscope is using light with a wavelength of 550 nm. Please help me break down and work through this practice question so that I can better under the steps and work it out on my own. 2. A microscope has a 1.1 cm diameter objective lens with a focal length of 2.3 mm. The microscope is using light with a wavelength of...
6. An optical fiber has an acceptance angle of 10.95 degrees. What's the numerical aperture for this fiber? A. 0.16 B. 1.02 C. 0.27 D. 0.19 7. An optical fiber has a core refractive index of 1.495 and a cladding refractive index of 1.47. What's the acceptance angle for this fiber? A. 13.11° B. 0.272° C. 9.79° D. 15.80° 8. While traveling through a cable, a signal loses 18.7 percent of its original power. What's this loss expressed in dB?...