Question

Phosphate buffer (pH range 5.8 – 8.0). Assume you have prepared two separate stock solutions: Solution...

Phosphate buffer (pH range 5.8 – 8.0). Assume you have prepared two separate stock solutions:

Solution A: 0.1M solution of monobasic potassium phosphate (KH2PO4) and

Solution B: 0.1M solution of dibasic potassium phosphate (K2HPO4)

The equilibrium: H2PO4 - H+ + HPO4 2-; pKa= 6.86 In order to get 200 mL of the desired buffer, you take 50 mL of solution A, add to it some amount of solution B, and then adjust the total volume to 200 mL by adding pure water.

** What will be the pH if you mix 50 mL of both solutions A and B?

0 0
Add a comment Improve this question Transcribed image text
Know the answer?
Add Answer to:
Phosphate buffer (pH range 5.8 – 8.0). Assume you have prepared two separate stock solutions: Solution...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions
  • In our experiment, we will be using a portion of the phosphate buffer system that is...

    In our experiment, we will be using a portion of the phosphate buffer system that is based upon the following equilibrium: H2PO4- HPO42- + H+ pKa = 7.2 In this case, H2PO4- will act as the acid and HPO42- will act as the base. Materials: 1M NaOH: 40.01 g/L of solution 1M HCl: 83 mL conc. HCl/L of solution Potassium phosphate, dibasic, K2HPO4, MW= 174.18 Potassium phosphate, monobasic, KH2PO4 MW= 136.09 **I already preformed this lab, but I struggled a...

  • Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. The buffer helps...

    Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. The buffer helps to maintain a constant pH. The osmolality and ion concentrations of the solution usually match those of the human body. A) You need to prepare a stock solution at pH 7.00 with KH2PO4 and Na2HPO4 (pKa =7.21). What would be the respective concentration of these substances if you wish to obtain the final phosphate concentration [HPO4 −2 ] + [H2PO4 − ] = 0.3...

  • Calculate how you will prepare 0.1M buffer solutions ranging from pH 1-12 in increments of 1...

    Calculate how you will prepare 0.1M buffer solutions ranging from pH 1-12 in increments of 1 pH unit. NaOH and HCl droppers will be available to make adjustments to the final pH. a. pH 9 Tris Base (pKa 8.08, 121.14g/mol) and Tris-HCl (151.59g/mol) are available. b. pH 10, 11, 12. CAPS (3-(Cyclohexylamino)-1-propanesulfonic acid, 221.32g/mol, pKa 10.4) is available. c. pH 7, 8. Dibasic potassium phosphate (pKa 6.82, 174.18g/mol) and monobasic potassium phosphate (136.09g/mol) are available

  • The Henderson-Hasselbalch equation relates the pH of a buffer solution to the pKa of its conjugat...

    The Henderson-Hasselbalch equation relates the pH of a buffer solution to the pKa of its conjugate acid and the ratio of the concentrations of the conjugate base and acid. The equation is important in laboratory work that makes use of buffered solutions, in industrial processes where pH needs to be controlled, and in medicine, where understanding the Henderson-Hasselbalch equation is critical for the control of blood pH. Part A As a technician in a large pharmaceutical research firm, you need...

  • Henderson-Hasselbach equation: pH- pKa log (IA-|/IHA]) 1. Phosphate buffer is a mixture of KH2PO4 and K2HPO4....

    Henderson-Hasselbach equation: pH- pKa log (IA-|/IHA]) 1. Phosphate buffer is a mixture of KH2PO4 and K2HPO4. Note that KH2PO4 has one additional proton. The pKa of the acid is 6.8. Use the Henderson-Hasselbach equation (above) to calculate the ratio of [K2HPO41[KH2PO4] needed to make a solution that is pH 7.2 2. To make a solution that is 0.2 M phosphate, the concentration of KH2PO4 and K2HPO4 must add up to 0.2 M. Use the ratio you calculated above, and the...

  • As a technician in a large pharmaceutical research firm, you need to produce 200. mL of...

    As a technician in a large pharmaceutical research firm, you need to produce 200. mL of a potassium dihydrogen phosphate buffer solution of pH = 7.07. The pKa of H2PO4− is 7.21. You have the following supplies: 2.00 L of 1.00 M KH2PO4 stock solution, 1.50 L of 1.00 M K2HPO4 stock solution, and a carboy of pure distilled H2O. How much 1.00 M KH2PO4 will you need to make this solution? (Assume additive volumes.)

  • Goal- to make a 1L solution of phosphate buffer at pH 7.8 If you make up...

    Goal- to make a 1L solution of phosphate buffer at pH 7.8 If you make up a 1M aqueous solution each of: NaH2PO4 and Na2HPO4 How much of each solution would you need to add (mix) to make up a 1 liter solution of 0.4 M phosphate at pH 7.8 Hint: the pKa for H2PO4- <-> HPO4 2- + H+ is 6.8)

  • As a technician in a large pharmaceutical research firm, you need to produce 450.ml of 1.00M potassium phosphate buffer...

    As a technician in a large pharmaceutical research firm, you need to produce 450.ml of 1.00M potassium phosphate buffer solution of pH = 7.13. The pKa of H2PO4 is 7.21. You have the following supplies: 2.00l of 1.00 KH2PO4 stock solution, 1.50 L of 1.00M KH2PO4 stock solution, and a carboy of pure distilled H2O How much 1.00 M KH2PO4 will you need to make this solution?

  • Sample Buffer Calculation: Given a pH of 5.4, prepare a buffer using one of the buffer...

    Sample Buffer Calculation: Given a pH of 5.4, prepare a buffer using one of the buffer systems below: System A: 3.5M acetic Acid (MM=60.1) : sodium acetate, anhydrous (MM=82.0) pKa = 4.74 System B: Sodium phosphate, monobasic (NaH2PO4 x H2O) (MM=138.0) : Sodium phosphate, dibasic, Na2HPO4 (MM=142.0) pKa = 6.86 a) Choose the buffer system most effective at pH of 5.4 b) Calculate the amounts of weak acid and conjugate base you need to make 25mL of 0.25M solutions (one...

  • As a technician in a large pharmaceutical research firm, you need to produce 200. mL of...

    As a technician in a large pharmaceutical research firm, you need to produce 200. mL of 1.00 mol L−1 potassium phosphate buffer solution of pH = 7.01. The pKa of H2PO4− is 7.21. You have the following supplies: 2.00 L of 1.00 mol L−1 KH2PO4 stock solution, 1.50 L of 1.00 mol L−1 K2HPO4 stock solution, and a carboy of pure distilled H2O. How much 1.00 mol L−1 KH2PO4 will you need to make this solution? Express your answer to...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT