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1. Identify the problem with this statement: PCR-based systems often yield results for a tiny sample...

1. Identify the problem with this statement: PCR-based systems often yield results for a tiny sample of relatively poor-quality DNA?

2. Name one type of cell in the human body from which you could NOT extract chromosomal DNA and explain why.

3. The final result in RFLP analysis looks like a simplified supermarket bar code. The latter is absolutely unique. Is the RFLP pattern unique?

4. The final result in the PM + DQA1 test is seen as blue dots on a white strip. Why are the positive and negative controls so important in this test?

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Answer #1

1. PCR is polymerase chain reaction. It is used to amplify a DNA sample.

PCR produces good quality DNA.

So, the correction will be good quality DNA.

PCR-based systems often yield results for a tiny sample of relatively good-quality DNA

2. The cell from which chromosomal DNA cannot be extracted is RBC. As, RBC lacks nucleus. When RBCs mature they lose their nucleus.

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