Do the titration on Unknown 6 (results are a pH of 13.75 after titration)
Give the:
3 letter abbreviation of the amino acid
pka1 (COOH) value?
pka2 (NH3+) value?
pka3 (side chain) value?
pI value?
The only info given is the ph that is 13.75 and 34 ml of titrant was used.
Since the pH of the solution is 13.75 after titration, it indicates that base has been used to titrate the solution and after titration, the resulting pH has come because of addition of some extra base.
Now among the amino acids, arginine which has the following structure has pKa3 at 12.48. Hence for the titration fo this amino acid, high concentration of base will be required. Therefore the possible amino acid is arginine.
The pKa1 for this is 2.17, the pka2 is 9.04 and pka3 is 12.48. This is known from literature.
Do the titration on Unknown 6 (results are a pH of 13.75 after titration) Give the:...
2. Soda has a pH between 3 and 4. Soda makers often add citric acid to give a tart taste to soda. 20 ml of 0.1 M citric acid (H 3C6H57, pa 3.1, pKa2 4.7, pKa3 6.4) is titrated with 0.1 M NaOH a2 4.7, pka3 6.4) is titrated pH 0 10 20 30 40 50 60 volume of NaOH Citric acid can be used as a buffer. The formula and charge of citric acid (H3CsH5o7, H2C6Hso, HCGH,o, CH3) depends...
Experimental Biochemistry pKa of Amino Acids.. ** PLEASE ANSWER IN CLER HAND WRITING PLEASE HELP AND ANSWER AS SOON AS POSSIBLE** 1- where the pKas are located (for pKa1, pKa2 and if applicable, pKa3) for your unknown. 2-write their values on the correct parts of the graph. c) mark where the buffering regions are. 3-Record the pKa values below, that you obtained from the graphpKa1=_______ pKa2=__________ ; pKa3 (if present)=_________ 4-For pI value: Show the correct formula to use for...
2. Soda has a pH between 3 and 4. Soda makers often add citric acid to give a tart taste to soda. 20 ml of 0.1 M citric acid (H 3CGH5 with 0.1 M NaOH pKa1 3.1, pKa2 4.7, pKa3 6.4) is titrated pH 0 10 20 30 40 50 60 volume of NaOH Citric acid can be used as a buffer. The formula and charge of citric acid (H3CHso7, H2CsH,0, H,o,2, CgH,o,3) depends on the pH. d. What is...
Using this titration graph (pH vs. base volume added), find the unknown Amino Acid and show its pK1, pK2 and pI. Unknown #3 buffer-pH vs. mL 0.5M NaOH 12 10 6 4 0 10 15 20 25 30 Volume of 0.5M NaOH (mL)
I have attached a titration of a mock amino acid with OH-. I have labeled the different charge states of the amino acid as I, II, III, IV. I have labeled different points on the titration curve as A, B, ....G. I want you to tell me which form(s) of the species I, II, III or IV predominates (is present at greatest concentration) at each of the lettered points on the curve. I would also like you to calculate the...
it's a bio-chem problem. "I have attached a titration of a mock amino acid with OH-. I have labeled the different charge states of the amino acid as I, II, III, IV. I have labeled different points on the titration curve as A, B,...G. I want you to tell me which form(s) of the species I,II,III,IV predominates (is present at greatest concentration) at each of the lettered points on the curve. I would also like you to calculate the PI...
it's a bio-chem problem. "I have attached a titration of a mock amino acid with OH-. I have labeled the different charge states of the amino acid as I, II, III, IV. I have labeled different points on the titration curve as A, B,...G. I want you to tell me which form(s) of the species I,II,III,IV predominates (is present at greatest concentration) at each of the lettered points on the curve. I would also like you to calculate the PI...
It's a weak acid strong base titration Experiment 4: Identification of an unknown acid by titration Page 2 of 15 Background In this experiment, you will use both qualitative and quantitative properties to determine an unknown acid's identity and concentration. To do this analysis, you will perform a titration of your unknown acid sample-specifically a potentiometric titration where you use a pH meter and record pH values during the titration, combined with a visual titration using a color indi- cator...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not migrate in an electric field. Since the peptide is zwitterionic, there are the same number of positive charges as negative charges on the peptide population. The pI can be estimated fairly accurately (within 0.1 or 0.2 pH units) from the pK values of all the proton dissociable groups in the peptide. Using pK values from the table at the right, estimate the pI value...
1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCl stock solution. In your response to this question, be very specific about the quantities of stock solution and deionized water to be used in the dilution and the...