Which technique is the most commonly employed for the detection of malonaldehyde formation? a) Comet assay...
Which technique is the most commonly employed for the detection of malonaldehyde formation?
a) Comet assay
b) TUNEL assay
c) Griess assay
d) Thiobarbituric acid-reactive substance assay
e) EPR spin trapping
Homework Answers
Solution :
Option d is correct option
technique is the most commonly employed for the detection of malonaldehyde formation is Thiobarbituric acid-reactive substance assay (TABS).
TBARS is probably the oldest and one of the most widely used assays for measuring lipid peroxidation end product malondialdehyde, Although MDA absorbs at 245 nm and 267 nm in acidic and basic solutions respectively, the most common method for detection is by derivatization to produce a fluorophore, in particular through the thiobarbituric acid assay.
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