The average molecular weight of a nucleotide (base + deoxyribose
+ 1 phosphate group) in DNA is 308g/mol of 308 Daltons (Da). This
number is the average molecular weight of
A = 312.2 g/mol
G = 328.2 g/mol
C = 288.2 g/mol T = 303.2 g/mol
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R-plasmid was used for the transformation experiment
a.What is the size of this plasmid (in kilo base pairs; kbp)?
How many bases is this?
b. What is the molecular weight of one plasmid molecule?
c. You used 25μl of 1ng/μl plasmid DNA per transformation. How many
grams of plasmid DNA
did you use? How many moles of plasmid DNA is that? How many
molecules of plasmid DNA
is that?
d. Assuming each transformed cell pick up one plasmid molecules
during the transformation
process, what percentage of DNA molecules successfully entered and
replicated inside the
competent cells?
The average molecular weight of a nucleotide (base + deoxyribose + 1 phosphate group) in DNA...
please answer as many questions with detail. 1. Evaluate how much "weight" (mass, strictly speaking) an average human loses daily due to gas exchange while breathing. Find all necessary data, indicate your sources, and present a detailed calculation. 2. 10.00 g of a Mg-Al alloy completely dissolved in hydrochloric acid. The volume of hydrogen gas given off in the chemical reaction is 12.29 L Find the mass composition (in mass %) of the alloy. Assume that the volume of 1...