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It is best to answer Question 1 and Question 2 to do this Question 3 although...

It is best to answer Question 1 and Question 2 to do this Question 3 although it is possible you can still get a correct answer if your answer to Question 2 is close.

The answer to question 2 was a pH of 2.25 and the answer to question 1 was that the general surface charge for the silica stationary phase is close to neutral.

Knowing the charge (or lack thereof) of the TLC stationary phase and the solution pH, what is the expected order of the following amino acids in terms of Rf (highest Rf is on the left, lowest Rf is on the right).

A D E I K Q

Put your answer as

"ADEIKQ" but in a different order.

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