Question

Rate (0.025, 0.03, 0.0325, 0.0550, 0.0725)

Substrate(10, 25, 50, 90, 150) it's in mM

Enzyem is Invertase, sucrose is substrate, DNS is used as indicator to measure ABS/OD. Professor said Km = 51.5 and Vm = 0.0739. Can you plot the Michaelis Menten and line-weaver burk and calculate the Vm and Km from each method. So far I can't get the data to match up. I can add the regression equation and absorbance for a standard glucose curve if needed.

Answer 1

When you are drawing any graph then you have to join all the points in such a way that the line passes through maximum points you don't have to join all the dots line to line.

It should be noted that LINEWEAVER BURK EQUATION has been derived from MICHAELIS MENTEN EQUATION by reciprocaling it.

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