How does surface plasmon resonance (SPR) detect proteins adsorbing to surfaces?
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How does surface plasmon resonance (SPR) detect proteins adsorbing to surfaces?
12. For surface plasmon resonance (SPR), answer the following questions: a) Describe the experimental set-up of the Kretschmann configuration to excite a surface plasmon in a metal thin film? b) Draw the experimental set-up, how does the laser beam interact with the metal thin film, what is its polarization etc. label everything clearly c) For the case of Gold thin film with ε--10 with air as the dielectric medium ε-1, if the prism has an index of refraction n-1.6 at...
12. For surface plasmon resonance (SPR), answer the following questions a) Describe the experimental set-up of the Kretschmann configuration to excite a surface plasmon in a metal thin film? b) Draw the experimental set-up, how does the laser beam interact with the metal thin film, what is its polarization etc. label everything clearly. c) For the case of Gold thin film with ε :10 with air as the dielectric medium ε-1, if the prism has an index of refraction n-1.6...
You can solve only c) or d) ? For surface plasmon resonance (SPR), answer the following questions: a) Describe the experimental set-up of the Kretschmann configuration to excite a surface plasmon in a metal thin film? b) Draw the experimental set-up, how does the laser beam interact with the metal thin film, what is its polarization etc. label everything clearly. c) For the case of Gold thin film with ε--10 with air as the dielectric medium ε-1, if the prism...
Using a technique called surface plasmon resonance, the on-rate for the transcription factor to bind DNA is measured to be 3.1 ´ 107 M-1 s-1, while the off-rate of the complex is 0.22 s-1. What is the change in standard Gibbs free energy for the binding reaction? (T = 25 °C)
A membrane is being engineered for filtering proteins. Its pores have charged surfaces, so some of the sieving effect of the membrane comes from the electrostatic repulsion of the proteins from the walls of the pore. Consider the membrane pore to be a parallel slit 10 nm in width and the surface potential to be 50 mV. Assume the potential at the midplane to be low, and calculate it for the case of 15 mM ionic strength (coming from a...
The folding of proteins is thermodynamically spontaneous. a. What does this mean? How is it defined? b. This spontaneity appears, on the surface, to be counterintuitive, because of the change in entropy, going from one state to another. Explain briefly. c. How is the apparent contradiction in part (b) overcome to ensure the spontaneity of the process?
Two equipotential surfaces surround a +170 C point charge. How far is the 190-V surface from the 75.0-V surface?
Two equipotential surfaces surround a +3.90x10^-8 -C point charge. how far is the 150-V surface from the 77.0-V surface?
Two equipotential surfaces surround a +2.10 x 10-8-C point charge. How far is the 140-V surface from the 46.0-V surface?
Two equipotential surfaces surround a +3.90 x 10-8-C point charge. How far is the 240-V surface from the 62.0-V surface?