Using a technique called surface plasmon resonance, the on-rate for the transcription factor to bind DNA...
Using a technique called surface plasmon resonance, the on-rate for the transcription factor to bind DNA is measured to be 3.1 ´ 107 M-1 s-1, while the off-rate of the complex is 0.22 s-1. What is the change in standard Gibbs free energy for the binding reaction? (T = 25 °C)
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Using a technique called surface plasmon resonance, the on-rate
for the transcription factor to bind DNA...
32P-labeled promoter DNA was mixed, in various combinations, with the general transcription factors TFIID (D), TFIIA (A...
32P-labeled promoter DNA was mixed, in various combinations, with the general transcription factors TFIID (D), TFIIA (A), TFIIB (B), TFIIF (F), and RNA polymerase II (Pol II). Binding of these factors to the promoter DNA was autoradiograph of the gel is shown below. The anode (+ electrode) is at the bottom. Labels on top of each lane indicate the factors mixed with DNA. For binding reactions 3-7 (lanes 3-7), the concentration of RNA Pol II was continually increased from zero...
Need help on these questions ? 1.) Steroid hormones trigger transcription of target genes by... ...
Need help on these questions ? 1.) Steroid hormones trigger transcription of target genes by... a. activating the release of Ca+2 ions that bind to and activate transcription factors. b. activating a kinase cascade that results in the activation of a transcription factor for a set of genes. c. binding directly to DNA and stimulating transcription. d. binding to nuclear receptors that act as transcription factors for specific genes. 2.) Which type of cell-surface receptor(s), when...
You are using a ChIP assay to study the positioning of a DNA-binding transcription factor (Dbp1p)...
You are using a ChIP assay to study the positioning of a
DNA-binding transcription factor
(Dbp1p) on transcribed genes. You fragment DNA from wild type
cells and immunoprecipitate
Dbp1p with an antibody to it. Then you PCR amplify the DNA
associated with Dbp1p using
different sets of primers for a particular gene (G3PD). One set
of primers was specific for the
TATA box region of this gene, (lane 1) another pair of primers
amplified the 3’ end of the open...
ch 14 answer each of these. answer all 3 please HI HCI reactants Reaction intermediates are...
ch 14 answer each of these.
answer all 3 please
HI HCI reactants Reaction intermediates are species that are formed in one step of a mechanism and consumed in another step. For example, can act as an intermediate in the following reaction: H2(g) + 2Cl(g) + 2HCl(g) + 12(g). You can often express the concentration of in terms of the concentrations of the of the overall reaction to be consistent with the experimentally observed rate law as the overall reaction...
please help!! transcription? How could the presence of a hormone in the blood have an effect...
please help!!
transcription? How could the presence of a hormone in the blood have an effect on this structure? 6 points 3. What is an enhancer region and why is it important in the regulation of eukaryotic Contents Cancer Genes that cause cancer are called oncogenes. d. promoter genes. a. operator genes. b. pseudogenes.c 2A mutation in which of the following genes would be LEAST likely lead to a cancer? a. growth hormone gene b. growth hormone receptor gene c....
6. What does a buffer do? Why are bicarbonate and phosph Henderson Hasselbalch equation to calculate...
6. What does a buffer do? Why are bicarbonate and phosph Henderson Hasselbalch equation to calculate pH if pKa i ate really good biological buffers? Use the s 4.0, [A-1-10μΜ, and [HA-1000μΜ 7. In what eukaryotic cell structure (cytosol, nucleus, mitochondrion, inner mitochondrial membrane, rough ER, smooth ER, plasma membrane etc.) do the following functions take place? Transcription Translation (2 sites) Glycolysis Kreb's Cycle Electron transport chain AG, of a reaction is +11,600 The standard J/mol. What is K at...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the Unit gone and inserted in rat zygotes (by microinjection) a recombinant construct containing the Uni-1 coding sequence its entire 5 UTR region, covering both the minimal promoter and a series of cutative regulatory als elements. The mutantrats develop glorious, luminous hair. Hagrid has also discovered that the UNI-1 protein binds very tightly to DNA and hypothesizes it is a transcription factor that regulates proliferation...
Question 1 of 8 2.0 Points Which of the following statements about the cytoskeleton is false?...
Question 1 of 8 2.0 Points Which of the following statements about the cytoskeleton is false? A. The cytoskeleton is made up of three types of protein filaments. B. The cytoskeleton controls the location of organelles in eukaryotic cells. C. Covalent bonds between protein monomers hold together cytoskeletal filaments. D. The cytoskeleton of a cell can change in response to the environment. Reset Selection Question 2 of 8 2.0 Points Which of the following statements about the function of the...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein intake. Little is lost from the body by excretion. It is catabolized in various tissues, where it is taken up by cells by pinocytosis. Albumin has a vast capacity for ligand binding due to the large number of charges on each molecule as well as the very large number of molecules available. This binding property of albumin is related to one of its functions,...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein...
INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein intake. Little is lost from the body by excretion. It is catabolized in various tissues, where it is taken up by cells by pinocytosis. Albumin has a vast capacity for ligand binding due to the large number of charges on each molecule as well as the very large number of molecules available. This binding property of albumin is related to one of its functions,...
32P-labeled promoter DNA was mixed, in various combinations, with the general transcription factors TFIID (D), TFIIA (A), TFIIB (B), TFIIF (F), and RNA polymerase II (Pol II). Binding of these factors to the promoter DNA was autoradiograph of the gel is shown below. The anode (+ electrode) is at the bottom. Labels on top of each lane indicate the factors mixed with DNA. For binding reactions 3-7 (lanes 3-7), the concentration of RNA Pol II was continually increased from zero...
You are using a ChIP assay to study the positioning of a
DNA-binding transcription factor
(Dbp1p) on transcribed genes. You fragment DNA from wild type
cells and immunoprecipitate
Dbp1p with an antibody to it. Then you PCR amplify the DNA
associated with Dbp1p using
different sets of primers for a particular gene (G3PD). One set
of primers was specific for the
TATA box region of this gene, (lane 1) another pair of primers
amplified the 3’ end of the open...
ch 14 answer each of these.
answer all 3 please
HI HCI reactants Reaction intermediates are species that are formed in one step of a mechanism and consumed in another step. For example, can act as an intermediate in the following reaction: H2(g) + 2Cl(g) + 2HCl(g) + 12(g). You can often express the concentration of in terms of the concentrations of the of the overall reaction to be consistent with the experimentally observed rate law as the overall reaction...
please help!!
transcription? How could the presence of a hormone in the blood have an effect on this structure? 6 points 3. What is an enhancer region and why is it important in the regulation of eukaryotic Contents Cancer Genes that cause cancer are called oncogenes. d. promoter genes. a. operator genes. b. pseudogenes.c 2A mutation in which of the following genes would be LEAST likely lead to a cancer? a. growth hormone gene b. growth hormone receptor gene c....
6. What does a buffer do? Why are bicarbonate and phosph Henderson Hasselbalch equation to calculate pH if pKa i ate really good biological buffers? Use the s 4.0, [A-1-10μΜ, and [HA-1000μΜ 7. In what eukaryotic cell structure (cytosol, nucleus, mitochondrion, inner mitochondrial membrane, rough ER, smooth ER, plasma membrane etc.) do the following functions take place? Transcription Translation (2 sites) Glycolysis Kreb's Cycle Electron transport chain AG, of a reaction is +11,600 The standard J/mol. What is K at...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the Unit gone and inserted in rat zygotes (by microinjection) a recombinant construct containing the Uni-1 coding sequence its entire 5 UTR region, covering both the minimal promoter and a series of cutative regulatory als elements. The mutantrats develop glorious, luminous hair. Hagrid has also discovered that the UNI-1 protein binds very tightly to DNA and hypothesizes it is a transcription factor that regulates proliferation...
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