ZuoTi.Pro
Question

A final step in the analysis of enzymatic activity during practicals 3 and 4 on was...

A final step in the analysis of enzymatic activity during practicals 3 and 4 on was the addition of NaOH to your samples of acid phosphatase . In less than 100 words provide two reasons why you added NaOH to measure light absorbance of the samples explaining the molecular effects that NaOH had in each case! (0.5 mark)

science   chemistry   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Request Professional Answer

Request Answer!

We need at least 10 more requests to produce the answer.

0 / 10 have requested this problem solution

The more requests, the faster the answer.

Request! (Login Required)

All students who have requested the answer will be notified once they are available.
Know the answer?
Add Answer to:
A final step in the analysis of enzymatic activity during practicals 3 and 4 on was...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Similar Homework Help Questions

  • 3. The spectrophotometric analysis done in this activity is measuring the absorbance of the base form...

    3. The spectrophotometric analysis done in this activity is measuring the absorbance of the base form (blue form) of the indicator. The blue form absorbs light best at 615 nm (10-h(A) light; thus, the K, value was calculated using the equation K. = Ar-A for sorbance values measured at 615 nm. The derivation for why this equation is equal to the K is included in your Background Section--Spectrophotometric Method. (H,OA derive Starting from the equilibrium constant expression for a weak...

  • Oxalic addindate CH2OT-2H2 Fill out the table with the appropriate data from Part C, Trial 1...

    Oxalic addindate CH2OT-2H2 Fill out the table with the appropriate data from Part C, Trial 1 Trial 2 439 Mass of Oxalic Acid Dihydrate (g) Moles of Oxalic Acid Dihydrate (Convert grams of oxalic acid to moles using the molecular weight of oxalic acid dihydrate) Moles of NaOH (Convert moles of Oxalic Acid Dihydrate to moles of NaOH using the mole ratio from the reaction) 25mL 41.6mL 41.35 mL Initial burette reading (mL) 2omL 32.00ML Final burette reading (mL) Volume...

  • 4. Calculate the pH of your sarhple at several points during the titration. In each case, record your calcula...

    4. Calculate the pH of your sarhple at several points during the titration. In each case, record your calculated pH and your experimentally observed pH. Calculate the initial pH of your sample before any titrant was added. (Do not forget that you added some DI water to your sample before you began titrating.) Record your calculated pH value below and the experimentally observed value from your graph. a. Observed: Calculated: b. Calculate the pH after 7.00 mL of NaOH was...

  • grams of KHP 0.6413 mL NaOH needed to titrate KhP to endpoint 38.80 Grams of unknown...

    grams of KHP 0.6413 mL NaOH needed to titrate KhP to endpoint 38.80 Grams of unknown acid: 1.5106 mL NaOH needed to titrate unknown acid to endpoint 33.55 need to know molarity of NaOH solution and Molar mass of unknown acid. Please help and show work! CHEM 265 LAB EXPERIMENT NUMBER 4 DETERMINATION OF THE MW OF AN UNKNOWN ACID This experiment utilizes a titration to determine the molecular weight of an unknown acid. In this experiment, the endpoint of...

  • Experiment 6. Acid-Base Titration Name Person # Teaching Assistant Darc Section Code Data Sheet * Unknown...

    Experiment 6. Acid-Base Titration Name Person # Teaching Assistant Darc Section Code Data Sheet * Unknown is a Diprotic Acid Table 6.1. Mass and volume data for titration of primary standard acid and unknown acid with sodium hydroxide. M -1 Trial 1 T rial Trial 3 Mass of weighing paper (g) 0,3898 10.3794 0.4041 Mass of weighing paper and oxalic acid, H,C,0,2H,0 (g) 0.5828 10.5634 0.5947 Initial reading of buret (mL) 2.59 0.00 oslo Final reading of buret (mL) 24....

  • CHEM 242L Exp. 4 Report/ Synthesis of Oil of Wintergreen Name Score: 20 1) Assess the effectivene...

    please answer all these questions, thank you. CHEM 242L Exp. 4 Report/ Synthesis of Oil of Wintergreen Name Score: 20 1) Assess the effectiveness of the experiment and your technique by A) reporting the yield of each step in grams and percent as well as the overall percent yield (What is "overall percent yield?). SHOW ALL CALCULATIONS! B) Give a complete analysis of the identity and purity of your final product and any isolated intermediates 2 What was the purpose...

  • lab report titration help please! additional images on lab report 3. Federal standards require that any...

    lab report titration help please! additional images on lab report 3. Federal standards require that any commercial material called "vinegar" must contain as cast acetic acid. According to your results in question 4, does the vinegar sample you titrated meet the Federal standards? Explain why or why not. PART B: IDENTIFICATION OF AN UNKNOWN ACID DATA Molarity of NaOH used Mass of unknown acid used 0.050M 0.5989 14.43mc Average volume of NaOH used in the titration CALCULATIONS 1. Using the...

  • Help on prelab NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME...

    Help on prelab NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...

  • This is all the information I have. Any Help would be appreciated. Titration of an Antacid...

    This is all the information I have. Any Help would be appreciated. Titration of an Antacid (i.e., an anti-acid, in other words a base) Part A Procedure: 1. To a beaker of appropriate size add 190 mL of distilled water. Afterwards, using your 10 mL graduated cylinder, add 10 mL of 4 M HCl (hydrochloric acid) stock solution (CAUTION - STRONG ACID-CORROSIVE!) to the 190 mL of distilled water. Mix the solution well. Using a Rainin pipet, carefully pipet 10.000...

  • help on number 3 here is lab we did EXPERIMENT 19 An Analysis of Commercial Antacids...

    help on number 3 here is lab we did EXPERIMENT 19 An Analysis of Commercial Antacids Rolaids Alka Seltzer OBJECTIVES: Determine the per gram neutralizing ability of a commercial antacio Determine the per gram neutralizing ability of sodium bicarbonate Compare the effectiveness of several antacids. INTRODUCTION: "How do you spell relief two hecimie The parietal cells in the stomach secrete sufficient hydrochloric aci 0.10 M The flow of HCl increases when food enters the stomach and so acid secrete sufficient...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT