If dialysis is allowed to proceed to equilibrium before each change of dialysate buffer, the substances...
If dialysis is allowed to proceed to equilibrium before each change of dialysate buffer, the substances retained by the membrane are purified by a factor equal to the ratio of buffer volume to sample volume. If 5mL of a solution contains 1mol/L NaCl is placed in a dialysis membrane and then placed in 500 mL of water and allowed to reach equilibrium, what will the final concentration of NaCl be within the dialysis membrane? If the dialysis membrane above is removed and placed into a fresh 500 mL of water, what will the final concentration of NaCl be within the dialysis membrane after a second equilibration period?
Homework Answers
0.25mol/L NaCl will be retained
This process is based on osmosis and it will continue till equilibrium is acheived.
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If dialysis is allowed to proceed to equilibrium before each
change of dialysate buffer, the substances...
A. What is the Isoelectric point pI of the peptide Gly-Glu-Ala-Lys-Val ? B. A purified protein...
A. What is the Isoelectric point pI of the peptide Gly-Glu-Ala-Lys-Val ? B. A purified protein is in a Hepes buffer at pH 7 with 300 mM NaCl. A sample (2mL) of the protein solution is placed in a tube made of dialysis membrane and dialyzed against 1 L of the same Hepes buffer with no NaCl. Small molecules and ions (such as Na+, Cl-, and Hepes) can diffuse across the dialysis membrane, but the protein cannot. 1. Once the...
8) indicate if each statement below is true or false (one emror allowed) (4.5 points) a)...
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8) Indicate of each statemente are each statement below or lowed) (4.5 points in a 30.97g of phosphorous there are...
8) Indicate of each statemente are each statement below or lowed) (4.5 points in a 30.97g of phosphorous there are 6.022x10 toms of phosphorum b) Solubility of gases in water in outy of gases in water increase as the solution temperature increases c) Elements in group 2 show a tendency to lose 2 electrons and become positively charged d) Oxygen is more electronegative than sulfur e) When dissolving 9.0 g NaCl within one liter of water the solution is 0.9%...
A buffer solution is able to maintain a constant pH when small amounts of acid or...
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please , i need Write the protocol by your own words ( steeps) 5. Add 1...
please , i need Write the protocol by your own words (
steeps)
5. Add 1 volume of 70% ethanol to the cleared lysate, and mix immediately by pipetting. Do not centrifuge. Proceed immediately to step 6. Note: The volume of lysate may be less than 350 pl or 600 pl due to loss during homogenization and centrifugation in steps 3 and 4. Note: Precipitates may be visible after addition of ethanol. This does not affect the procedure. 6. Transfer...
It's a weak acid strong base titration Experiment 4: Identification of an unknown acid by titration...
It's a weak acid strong base titration
Experiment 4: Identification of an unknown acid by titration Page 2 of 15 Background In this experiment, you will use both qualitative and quantitative properties to determine an unknown acid's identity and concentration. To do this analysis, you will perform a titration of your unknown acid sample-specifically a potentiometric titration where you use a pH meter and record pH values during the titration, combined with a visual titration using a color indi- cator...
Exercise 2 Separation of a Mixture Based on Acid-Base Properties One purpose of this exercise is...
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Can you please explain in simple terms the methods of this experiment? I'm having difficulty visualizing...
Can you please explain in simple terms the methods of this experiment? I'm having difficulty visualizing and understanding what is being done. I have copied and pasted some of the text from the article. Thank you! Abstract : Obesity, high-fat diets, and subsequent type 2 diabetes (T2DM) are associated with cognitive impairment. Moreover, T2DM increases the risk of Alzheimer's disease (AD) and leads to abnormal elevation of brain beta-amyloid levels, one of the hallmarks of AD. The psychoactive alkaloid caffeine...
For the preparation and standardization of NaOH with KHP im supposed to boil water for 1hr and 30 min to remove CO2
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8) indicate if each statement below is true or false (one emror allowed) (4.5 points) a) In a 30.97g of phosphorous there are 6.022x10 atoms of phosphorous b) Solubility of gases in water increase as the solution temperature increases c) Elements in group 2A show a tendency to lose 2 electrons and become positively charged -2 d) Oxygen is more electronegative than sufur e) When dissolving 9.0 a NaCI within one liter of water the solution is 0.9% ) An...
8) Indicate of each statemente are each statement below or lowed) (4.5 points in a 30.97g of phosphorous there are 6.022x10 toms of phosphorum b) Solubility of gases in water in outy of gases in water increase as the solution temperature increases c) Elements in group 2 show a tendency to lose 2 electrons and become positively charged d) Oxygen is more electronegative than sulfur e) When dissolving 9.0 g NaCl within one liter of water the solution is 0.9%...
A buffer solution is able to maintain a constant pH when small amounts of acid or base are added to the buffer. Consider what happens when 1 mL of a 5 M solution is added or 0.005 mol of HCl are added to a 100.0 mL solution acetic acid buffer that contains 0.0100 mol of Acetic acid, HC,H,O,, and 0.0100 mol of sodium acetate, NaC,H,O,. The initial concentration of both the acid and the base are 0.0100 mol/ 0.1000 L...
please , i need Write the protocol by your own words (
steeps)
5. Add 1 volume of 70% ethanol to the cleared lysate, and mix immediately by pipetting. Do not centrifuge. Proceed immediately to step 6. Note: The volume of lysate may be less than 350 pl or 600 pl due to loss during homogenization and centrifugation in steps 3 and 4. Note: Precipitates may be visible after addition of ethanol. This does not affect the procedure. 6. Transfer...
It's a weak acid strong base titration
Experiment 4: Identification of an unknown acid by titration Page 2 of 15 Background In this experiment, you will use both qualitative and quantitative properties to determine an unknown acid's identity and concentration. To do this analysis, you will perform a titration of your unknown acid sample-specifically a potentiometric titration where you use a pH meter and record pH values during the titration, combined with a visual titration using a color indi- cator...
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