Question

ELISA is a

Answers: a. A technique that can detect and estimate the concentration of a protein by reacting with a primary antibody, then reacting with a secondary antibody containing a detection system. b. Mechanical separation of mixtures of cells based on their surface antigens. Antibodies with different fluorescent labels are used in the separation. c. The use of patient's serum to identify antibodies useful against various infectious diseases. d. Technique to identify proteins needed for growth of pathogens inside a host. Pathogen proteins are fused to a reporter such as GFP and then screened for growth inside the host. e. Methods used to count and measure various kinds of cells by using fluorescently-labeled antibodies.

C is incorrect.

Answer 1

A is correct answer because ELISA is a detection test in immunology in which primary antibody is bound with desired antigen or protein and then 2nd labelled antibody is bound with primary one. From labelled 2ndry antibody, detection of antigen is done as amount of antigen will be directly proportional to expression of fluorescent light released by labelled substrate.