How do the following techniques compare to the Lowry assay, in terms of their selectivity, specificity...
How do the following techniques compare to the Lowry assay, in terms of their selectivity, specificity and sensitivity?
- Biuret reaction
- CB dye-binding assay (Bradford assay)
comment on:
selectivity (does the assay give different results for different proteins)
specificity ( does the assay react only with the substance being measured)
sensitivity ( how much of the compound do you need to get a reliable measurement?)
use table below to answer question
| Sensitivity | Selectivity | Specificity | |
| Lowry assay | |||
| Bradford assay | |||
| Biuret reaction |
Homework Answers
Biuret method is highly specific does not react with other much while Lowry method has low specificity.
Bradford assay is amino acid composition dependent and very selective.
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