Question

To create a viral vector for delivery of genes into mammalian cells, the virus must first...

To create a viral vector for delivery of genes into mammalian cells, the virus must first be engineered to remove:

a. viral coat proteins.

b. all viral genes, replacing them with the mammalian genes to be delivered.

c. viral genes involved with virus replication and add mammalian genes to be delivered.

d. the viral genome and coat proteins and replace them with recombinant plasmids carrying the mammalian genes to be delivered.

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Answer #1

Answer is: c. viral genes involved with virus replication and add mammalian genes to be delivered.

Explanation:

To create a viral vector for delivery of genes into mammalian cells, the virus must first be engineered to remove replication genes and reintroduced separately to the cells.

To remove replication genes of the viral genome will ensure that cells cannot replicate and thus cannot infect the host cells. The cells which has reintroduced replication gene has viral genome to be duplicated and produce more of the viruses.



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