List and describe the factors that can affect enzymatic reactions.
a. Substrate Concentration
b. Enzyme Concentration
c. pH
d. Temperature
e. Cofactors
f. Inhibitors
(I have them listed but do not understand their affects)
List and describe the factors that can affect enzymatic reactions. a. Substrate Concentration b. Enzyme Concentration...
11. Local conditions can affect the specificity of an enzyme for its substrate, and thus the enzyme's catalytic activity. Which of the following alterations would be least likely to affect an enzyme in this manner? a) Increased temperature b) Increased substrate concentration c) increased H concentration d) Increased pH e) none of the above True / False: (3 pts each) 12. The Gl tracts of carnivores are longer and more specialized than those of herbivores. 13. Micelles are formed when...
3. Why is an allosteric enzyme more sensitive to substrate concentration around Km values than a Michaelis-Menten enzyme with the same Vmax? 4. Explain how pH and temperature influence enzyme activity. ( A Lineweaver-Burk (double reciprocal) plot was used to compare the effects of three different reversible inhibitors (A, B and C) on an enzyme. The plot of 1/V vs 1/[S] for the enzyme with no inhibitor is shown in a solid black line. The plot of 1/V vs 1/[S]...
Please answer all of those questions 7. Which of the followings is correct about the mechanisms of enzymatic reactions? General acid-base mechanism b. Metal elements mediated mechanism a. Covalent bonding mediated mechanism d. All above c. e. None a bove Which of the followings is correct about the meaning of the constant (Km) of Michaelis-Menten equation of enzyme catalyzed reactions? a. The constant and Michaelis-Menten equation is applicable only to the stage of reaction immediately after mixing of substrate(s) with...
The rate of an enzyme-catalyzed reaction initially increases with an increase in the substrate concentration, but eventually reaches a maximum value, even though the concentration of substrate continues to increase. Which of the following best explains why? O As substrate concentration increases, the substrates preferentially bind with each other instead of the active site of the enzyme, and no additional catalysis occurs. As substrate concentration increases, the active sites of all the enzyme molecules become occupied with substrate molecules, and...
Which of the following affects the Km? (There can be more than one answer) a. The enzyme concentration. b. The type of substrate. c. The substrate concentration. d. The temperature. e. The pH.
List 3 factors that affect the reaction rates of enzyme catalyzed physiological reactions.
2. hypothesize the best conditions (pH and temperature) under which the enzyme chymotrypsin functions with an appropriate reference. also, hypothesize what will occur in the presence of an inhibitor and the type of inhibition. EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
Please help with numbers 2 and 4 List three factors that affect enzyme activity. Draw a diagram illustrating the lock and key theory of enzyme activity. Describe two typed of enzyme inhibition. Describe how the pH optimum of an enzyme often to the pH of the environment within which it works.
What kind of kinetics is observed in an enzymatic reaction, under conditions where the substrate concentration is much higher with respect to KM ([S] >> KM)? Assume that a monomeric and non-allosteric enzyme is considered. A. Cooperative B. First Order C. Zero Order D. The systerm is at equilibrium and no reaction occures E. Second Order
Part A - Overview of enzyme structure and enzymatic reactions Enzymes are large globular proteins. Much of their three dimensional shape is the result of interactions between the R (variable) groups of their amino acids. The active site is the portion of the enzyme that will interact with the substrate the molecule that the enzyme acts upon. The nature and arrangement of amino acids in the active site make each enzyme specific to a substrate and to the reaction it...