A bilirubin standard of 5.0mg/dL had an absorbance of 0.898 at 454 nm. A patient sample...
A bilirubin standard of 5.0mg/dL had an absorbance of 0.898 at 454 nm. A patient sample had an absorbance of 0.600 at 454nm in the same cuvette. What was the bilirubin concentration of the sample?
Answer is 3.3 mg/dL
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A bilirubin standard of 5.0mg/dL had an absorbance of 0.898 at
454 nm. A patient sample...
given the standard of 200 mg/dl and the absorbance of that standard is 0.640. What is...
given the standard of 200 mg/dl and the absorbance of that standard is 0.640. What is the concentration of the unknown if the absorbance is 0.252?
Post LaboratorY Qustions A colored salution had a large absorbance peak at 690 nm Use the...
Post LaboratorY Qustions A colored salution had a large absorbance peak at 690 nm Use the visitle spectrum in your text or other reliable source to determine the color of this solution 2 A soft drink had a tertrazine also known as yellow #5 concentration of 1.75 x 10 M If a person drank two 12 ounce cans of this beverage for a vear (365 days). What mass of tartrazine would the person consume? Moleculer weight 534 g/mol for tartrazine....
A Bill' external standard curve built from absorbance signal vs. analyte concentration (in uM) has the...
A Bill' external standard curve built from absorbance signal vs. analyte concentration (in uM) has the following calibration curve equation y = 0.0500 x + 0.003. If a sample produced an absorbance of 0.300, what was the Bi concentration in the solution in the cuvette? A. 0.015 mM B. 6.00 mm c. 5.94 mm D. 0.600 mm Reset Selection estion 4 of 5 2 Points If the concentration of Bi in a diluted sample (after dilution of the original sample)...
10.0 mg/dl calibration reference had an absorbance of 628 bance of .270. The 24 example: A...
10.0 mg/dl calibration reference had an absorbance of 628 bance of .270. The 24 example: A urinary calcium level (mg Cat+ /24 hours) for the following while a specimen urine diluted 1:2 gave an absor hour uri ne volume was 1320 ml. (Show all work) Is this value normal or abnormal? Explain. (6 points)
please help!! will give feedback! Examine the given Beer's law standard curve for an unknown dye...
please help!! will give feedback!
Examine the given Beer's law standard curve for an unknown dye measured in a 1.0 cm cuvette. Absorbance of Unknown Dye at 542 nm Absorbance at 542 nm y = 15200x -0.018 5.00E-06 1.00E-OS 1.50E-05 2.00E-05 2.50E-053.006-053.50E-OS Concentration (M) What is the concentration (in M) of a sample of the unknown dye with an absorbance of 0.25 at 542 nm? Answer:
Operating Wavelength : 458.7 nm Preparation of Standard Calibration Curve of [FeSCN]2+ Sample Number Absorbance Final...
Operating Wavelength : 458.7 nm Preparation of Standard Calibration Curve of [FeSCN]2+ Sample Number Absorbance Final Concentration of [FeSCN]2+(M) A 0.157 4.0E-5 B 0.272 8.0E-5 C 0.422 1.2E-4 D 0.615 1.6E-4 E 0.722 2.0E-4 Determination of [FeSCN]2+ in Equilibrium Mixtures Sample Number Absorbance 1 0.240 2 0.334 3 0.436 4 0.544 Determination of [FeSCN]2+ in equilibrium mixtures For Sample #1: Enter the initial concentration of Fe3+ Calculate the equilibrium concentration of Fe3+ Enter the initial concentration of HSCN Calculate the...
need help on these Suppose you obtained the following data from two standard solutions of known...
need help on these
Suppose you obtained the following data from two standard solutions of known concentrations using a UV-Vis spectroscope: Analysis # Conc. (mg/L) 8.00 20.00 Absorbance (at 255 nm) 0.160 0.390 What is the concentration (in mg/L) of an unknown solution with an absorbance of 0.200 at 255 nm 10 points)? The unknown solution contains the same analyte as the standard solution does (Assume a path length of 1cm). 10. You prepared a stock solution by dissolving a...
Data Results (show all work on following pages) Standard Dilutions A-E Solution Absorbance А B Concentration(mg/L)...
Data Results (show all work on following pages) Standard Dilutions A-E Solution Absorbance А B Concentration(mg/L) 0.005 0.001 0.002 0-003 0-004 C 1. 188 0.232 0.47 0.702 0.953 D E Graph of Absorbance versus Concentration (mg/L) 81 Show work for concentration values Winai is in equation for mine samaru curve from the repuri dain? Extraction of B-carotene Mass or carrot 5.49 Absorbance of carrot solution (diluted if 0.455 necessary) Concentration of carrot solution (diluted 0.00194 if necessary)(mg/L) Concentration of original...
(5 questions) Data: Consider the data below for a Beer's Law calibration curve for an aqueous...
(5 questions) Data: Consider the data below for a Beer's Law calibration curve for an aqueous orange nye solution that has mas 450 nm. The solutions used were translucent. The data was collected using a 1.20cm wide test tube cuvette, using 2 = 450 nm. Trial Concentration (M) Absorbance Percent Transmittance 1 0 .0030 0.153 70.3 0.0060 0.312 48.8 0.0090 0.465 34.3 0.0120 0.627 23.6 0.0150 0.771 16.9 For each of the following errors/changes, indicate whether a repeat of the...
I forgot to put down that the observance (=OD) at 280nm is 0.679A Experiment 8: Bradford...
I forgot to put down that the observance (=OD) at 280nm is
0.679A
Experiment 8: Bradford assay and UV-method for determination of protein concentrations Materials - An ice bucket with ice, your purified sample, three visible plastic cuvettes for Bradford assay, one UV plastic cuvette for UV-method, a piece of parafilm, a Vis-spec, a Lab Quest, Bradford reagent, white tape (share), a sharpie. Bradford assay (reference 21) 1. Add 990 uk Bradford assay reagent to a plastic cuvette 2. Blank...
Post LaboratorY Qustions A colored salution had a large absorbance peak at 690 nm Use the visitle spectrum in your text or other reliable source to determine the color of this solution 2 A soft drink had a tertrazine also known as yellow #5 concentration of 1.75 x 10 M If a person drank two 12 ounce cans of this beverage for a vear (365 days). What mass of tartrazine would the person consume? Moleculer weight 534 g/mol for tartrazine....
A Bill' external standard curve built from absorbance signal vs. analyte concentration (in uM) has the following calibration curve equation y = 0.0500 x + 0.003. If a sample produced an absorbance of 0.300, what was the Bi concentration in the solution in the cuvette? A. 0.015 mM B. 6.00 mm c. 5.94 mm D. 0.600 mm Reset Selection estion 4 of 5 2 Points If the concentration of Bi in a diluted sample (after dilution of the original sample)...
10.0 mg/dl calibration reference had an absorbance of 628 bance of .270. The 24 example: A urinary calcium level (mg Cat+ /24 hours) for the following while a specimen urine diluted 1:2 gave an absor hour uri ne volume was 1320 ml. (Show all work) Is this value normal or abnormal? Explain. (6 points)
please help!! will give feedback!
Examine the given Beer's law standard curve for an unknown dye measured in a 1.0 cm cuvette. Absorbance of Unknown Dye at 542 nm Absorbance at 542 nm y = 15200x -0.018 5.00E-06 1.00E-OS 1.50E-05 2.00E-05 2.50E-053.006-053.50E-OS Concentration (M) What is the concentration (in M) of a sample of the unknown dye with an absorbance of 0.25 at 542 nm? Answer:
need help on these
Suppose you obtained the following data from two standard solutions of known concentrations using a UV-Vis spectroscope: Analysis # Conc. (mg/L) 8.00 20.00 Absorbance (at 255 nm) 0.160 0.390 What is the concentration (in mg/L) of an unknown solution with an absorbance of 0.200 at 255 nm 10 points)? The unknown solution contains the same analyte as the standard solution does (Assume a path length of 1cm). 10. You prepared a stock solution by dissolving a...
Data Results (show all work on following pages) Standard Dilutions A-E Solution Absorbance А B Concentration(mg/L) 0.005 0.001 0.002 0-003 0-004 C 1. 188 0.232 0.47 0.702 0.953 D E Graph of Absorbance versus Concentration (mg/L) 81 Show work for concentration values Winai is in equation for mine samaru curve from the repuri dain? Extraction of B-carotene Mass or carrot 5.49 Absorbance of carrot solution (diluted if 0.455 necessary) Concentration of carrot solution (diluted 0.00194 if necessary)(mg/L) Concentration of original...
(5 questions) Data: Consider the data below for a Beer's Law calibration curve for an aqueous orange nye solution that has mas 450 nm. The solutions used were translucent. The data was collected using a 1.20cm wide test tube cuvette, using 2 = 450 nm. Trial Concentration (M) Absorbance Percent Transmittance 1 0 .0030 0.153 70.3 0.0060 0.312 48.8 0.0090 0.465 34.3 0.0120 0.627 23.6 0.0150 0.771 16.9 For each of the following errors/changes, indicate whether a repeat of the...
I forgot to put down that the observance (=OD) at 280nm is
0.679A
Experiment 8: Bradford assay and UV-method for determination of protein concentrations Materials - An ice bucket with ice, your purified sample, three visible plastic cuvettes for Bradford assay, one UV plastic cuvette for UV-method, a piece of parafilm, a Vis-spec, a Lab Quest, Bradford reagent, white tape (share), a sharpie. Bradford assay (reference 21) 1. Add 990 uk Bradford assay reagent to a plastic cuvette 2. Blank...
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