In setting up the tubes for the standard curve in the Biuret reaction, which of the...
In setting up the tubes for the standard curve in the Biuret reaction, which of the 3 components (BSA, water, Biuret reagent) must be pippeted as accurately as possible and which could vary slightly in volume (e.g use of an unwiped blowout pipe) provided that the same error is made throughout?
Homework Answers
We should pipette BSA accurately as if it is not pipetted accurately then there will be error when it will be read by spectrophotometer. The wrong O.D. will give a wrong result.which will affect the shape of the curve and will give a false reading of the sample when the graph will be plotted.
So, BSA should be pipetted properly.
Water will cause some dilution but will not have a deleterious effect on the standard curve.
Biuret reagent gives the color but main ingredient is BSA.
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In setting up the tubes for the standard curve in the Biuret
reaction, which of the...
PART A- Observe Normal Catalase Reaction To determine the volume of oxygen released in ml) during...
PART A- Observe Normal Catalase Reaction To determine the volume of oxygen released in ml) during the degradation of H202 by catalase, you will first do trials using the set-up in Fig. 1. 1. Place 10 ml of the 3% hydrogen peroxide solution into a clean bottle 2. Add quickly catalase containing solution. 3. What gas is being released? 4. Throughout this investigation you will estimate the rate of the reaction (by measuring the volume of water displaced by O2...
You find yourself setting up another Western blot, however. today you are preparing the samples t...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
CHEM LAB hello can someone help me come up with a procedure for this lab it...
CHEM LAB
hello can someone help me come up with a procedure for this
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it is an standarization of acid and base solution
• Each student will receive approximately 100 mL of an unknown HCl. The approximate concentration of HCl will be 0.1 M or 1.0 M. There will be a common container with NaOH. The approximate concentration of NaOH will be 0.1 M or 1.0 M. You will be given the approximate concentrations of NaOH and HCl on...
Grignard reagent THE GRIGNARD REAGENT-PREPARATION AND REACTION In this experiment, you will prepa...
i need help with the postlab questions please
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INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein...
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INTRODUCTION Albumin is synthesised in the liver at a rate that is dependent on dietary protein...
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Please type your answer so I can read it, and I just need help for question...
Please type your answer so I can read it, and I just
need help for question one ( Hypothesis ) don't worry about the
rest of them.
Experimental plan: You are provided with a standard solution of albumin, which is 60 g/L. This is known as the primary standard. Your task is, complete the table below to prepare 3 other standards with 15, 30 and 45 g/L concentration, respectively with the final volume of 1 mL. These are known as...
Question 1 (1 point) Using the solubility curve, at which temperature do KCl and KCIO, have...
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EXPERIMENT 9: ELECTROLYSIS AND AVOGADRO'S NUMBER QUESTIONS 1. What solution is used in the apparatus for this experiment? a. Would anything else work? b. If so, what are some examples? 2. What gas forms at the cathode in this experiment? 3. What gas forms at the anode in this experiment? 4. How is the temperature of each of the gases determined? EXPERIMENT ELECTROLYSIS & AVOGADRO'S NUMBER UUUUUUUUUUU INTRODUCTION Electrolysis is the process of causing a chemical...
PART A- Observe Normal Catalase Reaction To determine the volume of oxygen released in ml) during the degradation of H202 by catalase, you will first do trials using the set-up in Fig. 1. 1. Place 10 ml of the 3% hydrogen peroxide solution into a clean bottle 2. Add quickly catalase containing solution. 3. What gas is being released? 4. Throughout this investigation you will estimate the rate of the reaction (by measuring the volume of water displaced by O2...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
CHEM LAB
hello can someone help me come up with a procedure for this
lab
it is an standarization of acid and base solution
• Each student will receive approximately 100 mL of an unknown HCl. The approximate concentration of HCl will be 0.1 M or 1.0 M. There will be a common container with NaOH. The approximate concentration of NaOH will be 0.1 M or 1.0 M. You will be given the approximate concentrations of NaOH and HCl on...
i need help with the postlab questions please
Grignard reagent THE GRIGNARD REAGENT-PREPARATION AND REACTION In this experiment, you will prepare a Grignard reagent, phenyl magnesium bromide, from bromobenzene and magnesium metal. The Grignard reagent will then react with methyl benzoate to form triphenylmethanol. Introduction Grignard reagents, such as organomagnesium halides, were discovered in 1910 by French chemist Victor Grignard. The Grignard reaction is one of the most general methods for carbon- carbon bond formation in all of organic chemistry....
Please type your answer so I can read it, and I just
need help for question one ( Hypothesis ) don't worry about the
rest of them.
Experimental plan: You are provided with a standard solution of albumin, which is 60 g/L. This is known as the primary standard. Your task is, complete the table below to prepare 3 other standards with 15, 30 and 45 g/L concentration, respectively with the final volume of 1 mL. These are known as...
Question 1 (1 point) Using the solubility curve, at which temperature do KCl and KCIO, have the same solubility? Solubility Curve Grams of solute/100g H30 to .- 30. 0 10 70 80 90 100 20 30 40 50 60 Temperature (°C) O 93°C 56°C O 85°C 0750 Previous Page Next Page Page 2 of 24 Question 2 (1 point) Saline (salt) solution is 0.9% by mass which is similar to tears, blood, and other body fluids. Which of the following...
need help with questions
EXPERIMENT 9: ELECTROLYSIS AND AVOGADRO'S NUMBER QUESTIONS 1. What solution is used in the apparatus for this experiment? a. Would anything else work? b. If so, what are some examples? 2. What gas forms at the cathode in this experiment? 3. What gas forms at the anode in this experiment? 4. How is the temperature of each of the gases determined? EXPERIMENT ELECTROLYSIS & AVOGADRO'S NUMBER UUUUUUUUUUU INTRODUCTION Electrolysis is the process of causing a chemical...
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