What would be the consequence of a mutation that prevented actin's ability to bind ATP with...
What would be the consequence of a mutation that prevented actin's ability to bind ATP with respect to the actin filament assembly/disassembly? Briefly explain.
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What would be the consequence of a mutation that prevented
actin's ability to bind ATP with...
answer only question number 7.please answer only question number 7 Why termination of a cell signaling...
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only question number 7.please answer only question number 7
Why termination of a cell signaling event is important? Explain with two examples how GPCR signaling is terminated. What is actin filaments treadmilling? What would be the consequence for actin filament assembly/disassembly if a mutation prevented actin's ability to bind ATP? What would be the consequence if a mutation prevented actin's ability to hydrolyze ATP? Predict the effect on a cell's actin cytoskeleton if function blocking antibody against cofilin was...
What would be the expected outcome of a mutation in E.coli that prevented methylation of adenine...
What would be the expected outcome of a mutation in E.coli that prevented methylation of adenine in the sequence 5'-GATC-3'? A) loss of epigenetic markers B) Randomization of mismatch repair C) increased mutation rate D) B and C E) All of these
A mutation in the operator of the trp operon prevents the repressor to bind to the...
A mutation in the operator of the trp operon prevents the repressor to bind to the operator, even in the presence of tryptophan. What is true with respect to the bacteria carrying this mutation? Select one: a. The bacteria will transcribe the genes of the trp operon even when tryptophan is present in the environment b. Tryptophan would not be able to bind to the repressor protein c. Small amounts of tryptophan will be lethal for the bacteria d. The...
According to the sliding filament model, binding sites on actin open when _, allowing the myosin...
According to the sliding filament model, binding sites on actin open when _, allowing the myosin head to bind. calcium ion levels fall creatine phosphate levels rise myosin levels rise calcium ion levels rise ATP levels rise Question 5 2 pts If cardiac muscle cells were prevented from undergoing aerobic metabolism, they ultimately would stop contracting undergo glycolysis synthesize ATP start contracting not be affected
An explanation would be appreciated. Thank you! Compared to the normal situation, in which actin monomers...
An explanation would be appreciated. Thank you!
Compared to the normal situation, in which actin monomers carry ATP, what do you predict would happen if actin monomers that bind a nonhydrolyzable form of ATP were incorporated into actin filaments? (a) Actin filaments would grow longer. (b) Actin filaments would grow shorter because depolymerization would be enhanced. (c) Actin filaments would grow shorter because new monomers could not be added to the filaments. (d) No change, as addition of monomers binding...
1) Why was the membrane incubated in blocking buffer? What would be the consequence of failing...
1) Why was the membrane incubated in blocking buffer? What would be the consequence of failing to block the membrane? 2) Explain the process of loading protein ladder and actin/myosin protein onto the gel with the fish samples and how it is necessary for analysis of the gel and immunoblot? 3) Why was a Bradford assay performed with the fish protein extracts? Fully explain why this was necessary for analysis of the gel and immunoblot? 4) The molecular mass of...
7. What would happen to the ATP yield of cellular respiration if (a) the transporter that...
7. What would happen to the ATP yield of cellular respiration if (a) the transporter that imports pyruvate into the mitochondria stopped working? Explain. (b) the transporter that imports pyruvate into the mitochondria worked only at 10% of its normal capacity? Explain. (c) The inner membrane of the mitochondria became affected by an antibiotic that prevented the buildup of a proton gradient. Explain. (d) ATP synthase started to make one ATP per proton. Explain.
4. You have a protein that needs to bind ATP to be functional and has a...
4. You have a protein that needs to bind ATP to be functional and has a KD for ATP of 10 μM. In you experiment your protein concentration is much less than that of ATP, and your final ATP concentration is 30 μM. A) What fraction of the protein is functional? Show your work. B) Suppose that you have a fluorescent ATP analog that can bind and activate your proteins that becomes quenched when it binds. Why would fluorescence change...
What is the consequence of a transition mutation in double-stranded DNA? A purine is replaced by...
What is the consequence of a transition mutation in double-stranded DNA? A purine is replaced by another purine, or a pyrimidine is replaced by another pyrimidine. A base pair is lost within the DNA of a gene, which causes a reading frameshift. The parent, but not its offspring, will be affected by the mutation. A base pair is added to the DNA within a gene, which causes a reading frameshift. A purine is replaced by a pyrimidine, or a pyrimidine...
What would be the consequence of the loss of the gene that encodes glycogenin in the...
What would be the consequence of the loss of the gene that encodes glycogenin in the liver? Liver glycogen will be less readily degraded. (The mobilization of liver glycogen will be impaired.) Glycogen will not be degraded unless a hormone induced pathway coverts the phosphorylase. Very little glycogen will be synthesized. Little glycogen would be present in the liver due to increased degradation. Which of the following reactions are nonspontaneous? FAD. 24" 2 FADH2 NADH + H → 20' NAD...
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only question number 7.please answer only question number 7
Why termination of a cell signaling event is important? Explain with two examples how GPCR signaling is terminated. What is actin filaments treadmilling? What would be the consequence for actin filament assembly/disassembly if a mutation prevented actin's ability to bind ATP? What would be the consequence if a mutation prevented actin's ability to hydrolyze ATP? Predict the effect on a cell's actin cytoskeleton if function blocking antibody against cofilin was...
A mutation in the operator of the trp operon prevents the repressor to bind to the operator, even in the presence of tryptophan. What is true with respect to the bacteria carrying this mutation? Select one: a. The bacteria will transcribe the genes of the trp operon even when tryptophan is present in the environment b. Tryptophan would not be able to bind to the repressor protein c. Small amounts of tryptophan will be lethal for the bacteria d. The...
According to the sliding filament model, binding sites on actin open when _, allowing the myosin head to bind. calcium ion levels fall creatine phosphate levels rise myosin levels rise calcium ion levels rise ATP levels rise Question 5 2 pts If cardiac muscle cells were prevented from undergoing aerobic metabolism, they ultimately would stop contracting undergo glycolysis synthesize ATP start contracting not be affected
An explanation would be appreciated. Thank you!
Compared to the normal situation, in which actin monomers carry ATP, what do you predict would happen if actin monomers that bind a nonhydrolyzable form of ATP were incorporated into actin filaments? (a) Actin filaments would grow longer. (b) Actin filaments would grow shorter because depolymerization would be enhanced. (c) Actin filaments would grow shorter because new monomers could not be added to the filaments. (d) No change, as addition of monomers binding...
What would be the consequence of the loss of the gene that encodes glycogenin in the liver? Liver glycogen will be less readily degraded. (The mobilization of liver glycogen will be impaired.) Glycogen will not be degraded unless a hormone induced pathway coverts the phosphorylase. Very little glycogen will be synthesized. Little glycogen would be present in the liver due to increased degradation. Which of the following reactions are nonspontaneous? FAD. 24" 2 FADH2 NADH + H → 20' NAD...
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