If you were to construct your own plasmid for a bacterium, what type of genes would it contain? Name at least 3.
For any one of these genes, what would be an advantage it would confer for this bacterium in the environment?
If you were to construct your own plasmid for a bacterium, what type of genes would...
Question 11 (1 point) Where would genes that confer antibiotic resistance in a bacterium be located? Genomic DNA Plasmid DNA Mitochondrial DNA Chloroplastic DNA Question 12 (1 point) Bacteria that release toxins after death should be treated with which type of antibiotic? Fungistats Bacteriostats Bacteriocides Fungicides Question 13 (1 point)
The plasmid pFR55 is a useful vehicle for the cloning of any DBA sequence in E. coli. A restriction map of pFR55 showing the restriction enzyme cutting sites is shown below. The plasmid can replicate I'm E. coli and carries the tetracycline resistance Tc and ampicillin resistance (Ap) genes for use as selectable markers in E. coli. a. you wish to insert a gene into the SalI site of pFR55. How would you select for E. Coli cells that have...
What type of environment do you think the lactase-persistent gene (genes code for proteins/enzymes) came from? In other words, what type of environment would be able to digest lactose be advantageous?
An F' plasmid from an exotic marine bacterium that can degrade petroleum when mated with an F-E. coli that cannot degrade petroleum results in an E. coli F' that can degrade petroleum. After sequencing the F' plasmid and comparing it to the F sequence, you see that it has about 6000 base pairs of additional DNA. You suspect that there is a gene in that sequence that encodes a protein that degrades petroleum. Describe how you would use reverse genetics...
How would you describe being "color-brave" in your own words? *How can you strive to be "color-brave?" Why is this important? *What are the hurdles in current business environment to overcome to be "color-brave?" *Why is diversity is a competitive advantage?
How would you characterize your own chances for a long life based on the genes you have inherited and on your health habits? Why? Put yourself in the role of an elderly person with symptoms of Alzheimer's disease. In spite of the fact that you have been a competent adult who has taken care of your family for decades, you no longer are able to perform tasks such as brushing your teeth or dressing yourself. How do you imagine you...
In your previous prac session you digested your POTC-A plasmid DNA with 3 enzyme mixes (AB and C). One mix contained the restriction endonuclease Kpnl alone, another contained both del and Not and another contained Sphi and Nhe, but you don't know which was which yet. The sites where these enzymes cut POTC-A are indicated on the plasmid map at the top of the page. Calculate the sizes of the DNA fragments that you would expect to see on your...
You are working with a plasmid the has a copy number of 3. What are the chances that one of the daughter cells (either one) will be cured of this plasmid (that is, will not have any plasmid in it?). Provide your answer as THE DENOMINATOR of the fraction such as "200" if the answer is 1/200, or "4" if the answer is 1/4.
What would you expect to find if you were to compare the number of genes present on the maternally and paternally inherited chromosomes 1? Explain.
1. If a restriction enzyme cuts a circular plasmid twice, how many fragments would you see on the gel? 2. How would you estimate the total number of base pairs in a plasmid by looking at the DNA fragments of the digested plasmid on a gel? 3. If a linear 1kb DNA fragment has a restriction site that is located 50 bp from one end of the plasmid, what would you expect to see if the digested and undigested DNA...