Question

The following is the recipe for 4X sample loading buffer:

4X stacking gel buffer – 10ml
10% SDS – 18 ml
Beta-mercaptoethanol – 2 ml
Glycerol – 20ml
Dash bromophenol blue (assume no volume change)

A) What is the final concentration of SDS in the solution of 4X sample loading buffer?

B) What is the purpose of the following reagents in 4X sample loading buffer:
i) SDS?
ii) Beta-mercaptoethanol?
iii) Glycerol?
iv) Bromophenol blue?

Answer 1