1. Medium and culturing of microorganisms - nutrients - growth factors  
1. Medium and culturing of microorganisms
- nutrients
- growth factors
- rich versus minimal medium
- agar: What is it? Why do we use it?
- sterilization (autoclave)
- aseptic technique: why do we do it?
Homework Answers
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1. Medium and culturing of microorganisms
- nutrients
- growth factors
 
1. The graph below depicts the growth of a Gram-positive, spore-forming bacterium Bacillus thuringiensis (B. t.). This species normally lives in the soil as well as the alkaline gut of insects. a)...
1. The graph below depicts the growth of a
Gram-positive, spore-forming bacterium Bacillus thuringiensis (B.
t.). This species normally lives in the soil as well as the
alkaline gut of insects.
a) Label on the graph the phases of this growth cycle.
Below, describe the important features of each phase.
b) The graph shown was obtained by culturing B. t. in
media containing only minimal amounts of nutrients and salts. Draw
a growth curve on the same graph that would...
What is the cleansing process that removes organic matcrial and soduces microorganisms to a safe level?...
What is the cleansing process that removes organic matcrial and soduces microorganisms to a safe level? a. Disinfection the sumbet of c. Sanitization d. Sterilization 151. Venipunctures or injections must be performed using which of the following protocast a. Asepsis b. Medical asepsis c. Surgical asepsis d. Sterile technique of the following aseptic procedures is performed on blood pressure cafts and sacthoncopes after each use? a. Sanitization b. Disinfection c. Sterilization d. Either A or B 153. Which of the...
Ubiquity of Microorganisms and Quadrant Streaking POST LAB DATE: SECTION: NAME: INSTRUCTOR: OULU Questions 1. in...
Ubiquity of Microorganisms and Quadrant Streaking POST LAB DATE: SECTION: NAME: INSTRUCTOR: OULU Questions 1. in microbiology lab, what is the function of an incubatore 2. Define: A. pure culture B. mixed culture C. Chromogenesis D. Enriched media E. Selective media F. Reducing media 3. What is the purpose of quadront streaking 4. Why should nutrient medium always be sterile before it is inoculated with a microbial specimen for culture and isolation 5. Why can't all microorganisms grow on a...
Think About It... 1. Speculate about the results of the following errors in aseptic i culturing...
Think About It... 1. Speculate about the results of the following errors in aseptic i culturing technique. Would pure culture be maintained? Would contamination occur? Why? • Placing caps on lab bench while performing transfer • Holding tubes at a 45° angle away from each other in the hand held in a TV shape Old culture tube is held in space nearest pointer in hand, rather than nearest ring finger: Tubes are held open while instrument is flamed: 2. What...
1-5 ASSESSMENT Critical Thinking and Learning Outcomes Review 1. In the streak-plate technique, how are microorganisms...
1-5
ASSESSMENT Critical Thinking and Learning Outcomes Review 1. In the streak-plate technique, how are microorganisms diluted and spread out to form indi lluted and spread out to form individual colonies? 2. Which area of a streak plate will contain the greatest amount of growth? The least amount of growth Explain your answers 3. Does each discrete colony represent the growth of one cell? Explain your answer. Why can a single colony on a plate be used to start a...
Please give me answers with details.. 1. Why should cotton provide an effective barrier against air...
Please give me answers with details..
1. Why should cotton provide an effective barrier against air contaminants, when the spaces between threads are relatively large? 2. What other ways might be used to close test tubes, still retaining a sound bacteriologic technique? 3. How would you protect culture medium from lint in a liter Erlenmeyer flask in which the cotton plug had to be large? 4. Explain your preference of method for the sterilization of : A hypodermic needle, rubber...
Effectiveness of Disinfectants Data Interpretation Please type the answers to the following questions below (double spaced)...
Effectiveness of Disinfectants Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. Answer the first 9 questions True or False 1. Disinfectants always remove all bacteria and fungal spores from an object. Antiseptic is another term for disinfectant. The presence of blood serum, skin tissue and other body fluids would interfere with the antimicrobial action of disinfectants. The use of carbolic acid as...
1. Human microbiota. What type of bacteria species are likely to be cultured from your throat...
1. Human microbiota. What type of bacteria species are likely to be cultured from your throat swabs on Blood agar? Why? 2. What is the purpose of the candle jar for the cultivation of the Blood Agar plate/throat swabs and what type of metabolism do you think you are targeting? 3.With reference to your two nasal swabs, what is the difference between the two culturing strategies: culture this swab on a mannitol-salt-agar plate and inoculate the nutrient broth containing 7.5%...
81 Exercise 7: Selective and Differential Growth Medium and Tests Questions 1. What elements are important...
81 Exercise 7: Selective and Differential Growth Medium and Tests Questions 1. What elements are important to consider when recording data about the growth of an organism? 2. What are some ways that cause the same bacteria to grow differently in different conditions? 3. List the types of growth patterns in broth. There are four dikprent type broth = Pellicle, Sediment, and flocculent ok growth uniform fine pattin in turbidity 4. List the types of growth patterns or colony morphology...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
1. The graph below depicts the growth of a
Gram-positive, spore-forming bacterium Bacillus thuringiensis (B.
t.). This species normally lives in the soil as well as the
alkaline gut of insects.
a) Label on the graph the phases of this growth cycle.
Below, describe the important features of each phase.
b) The graph shown was obtained by culturing B. t. in
media containing only minimal amounts of nutrients and salts. Draw
a growth curve on the same graph that would...
What is the cleansing process that removes organic matcrial and soduces microorganisms to a safe level? a. Disinfection the sumbet of c. Sanitization d. Sterilization 151. Venipunctures or injections must be performed using which of the following protocast a. Asepsis b. Medical asepsis c. Surgical asepsis d. Sterile technique of the following aseptic procedures is performed on blood pressure cafts and sacthoncopes after each use? a. Sanitization b. Disinfection c. Sterilization d. Either A or B 153. Which of the...
Ubiquity of Microorganisms and Quadrant Streaking POST LAB DATE: SECTION: NAME: INSTRUCTOR: OULU Questions 1. in microbiology lab, what is the function of an incubatore 2. Define: A. pure culture B. mixed culture C. Chromogenesis D. Enriched media E. Selective media F. Reducing media 3. What is the purpose of quadront streaking 4. Why should nutrient medium always be sterile before it is inoculated with a microbial specimen for culture and isolation 5. Why can't all microorganisms grow on a...
Think About It... 1. Speculate about the results of the following errors in aseptic i culturing technique. Would pure culture be maintained? Would contamination occur? Why? • Placing caps on lab bench while performing transfer • Holding tubes at a 45° angle away from each other in the hand held in a TV shape Old culture tube is held in space nearest pointer in hand, rather than nearest ring finger: Tubes are held open while instrument is flamed: 2. What...
1-5
ASSESSMENT Critical Thinking and Learning Outcomes Review 1. In the streak-plate technique, how are microorganisms diluted and spread out to form indi lluted and spread out to form individual colonies? 2. Which area of a streak plate will contain the greatest amount of growth? The least amount of growth Explain your answers 3. Does each discrete colony represent the growth of one cell? Explain your answer. Why can a single colony on a plate be used to start a...
Please give me answers with details..
1. Why should cotton provide an effective barrier against air contaminants, when the spaces between threads are relatively large? 2. What other ways might be used to close test tubes, still retaining a sound bacteriologic technique? 3. How would you protect culture medium from lint in a liter Erlenmeyer flask in which the cotton plug had to be large? 4. Explain your preference of method for the sterilization of : A hypodermic needle, rubber...
Effectiveness of Disinfectants Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. Answer the first 9 questions True or False 1. Disinfectants always remove all bacteria and fungal spores from an object. Antiseptic is another term for disinfectant. The presence of blood serum, skin tissue and other body fluids would interfere with the antimicrobial action of disinfectants. The use of carbolic acid as...
81 Exercise 7: Selective and Differential Growth Medium and Tests Questions 1. What elements are important to consider when recording data about the growth of an organism? 2. What are some ways that cause the same bacteria to grow differently in different conditions? 3. List the types of growth patterns in broth. There are four dikprent type broth = Pellicle, Sediment, and flocculent ok growth uniform fine pattin in turbidity 4. List the types of growth patterns or colony morphology...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
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