Provide two limitations of the traditional combination of restriction endonuclease and ligase based cloning.
Provide two limitations of the traditional combination of restriction endonuclease and ligase based cloning.
Homework Answers
1. The traditional cloning method has slightly lower sequence independence. Some times when the cleavage in the internal locations is required, restriction endonuclease is unable to do that. Obtaining of computable overhangs is very difficult through this cloning.
2. Sequencing of accurate overhang sequence are error-prone, laborious and tedious.
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Provide two limitations of the traditional combination of
restriction endonuclease and ligase based cloning.
After a plasmid vector has been linearized using one restriction endonuclease, DNA Ligase cannot recircularize the...
After a plasmid vector has been linearized using one restriction endonuclease, DNA Ligase cannot recircularize the plasmid unless it contains an insert DNA fragment to be cloned True or False True False
1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences...
1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences in the PCR product most likely came from _______, and the restriction enzyme recognition sequences in the plasmid most likely came from ________. a. A multiple cloning site / the primers b. The primers / a multiple cloning site c. Both came from primers d. Both came from the multiple cloning site e. Naturally present in the gene of interest / the multiple cloning...
1.) If we mix two restriction fragments cut with the same restriction enzyme in the presence of DNA ligase, ATP and appropriate buffer, we will obtain... A) protein B) carbohydrate C) recombinant DNA...
1.) If we mix two restriction fragments cut with the same restriction enzyme in the presence of DNA ligase, ATP and appropriate buffer, we will obtain... A) protein B) carbohydrate C) recombinant DNA or plasmid D) pAMP
What are two (2) main limitations of empirically based software estimation models? Name three (3) such...
What are two (2) main limitations of empirically based software estimation models? Name three (3) such models. Outline three (3) particular limitations of function point based estimation.
What are two (2) main limitations of empirically based software estimation models? Name three (3) such...
What are two (2) main limitations of empirically based software estimation models? Name three (3) such models. Outline three (3) particular limitations of function point based estimation.
Explain two possible limitations associated with establishing internally consistent compensation systems. Based off of Strategic Compensation,...
Explain two possible limitations associated with establishing internally consistent compensation systems. Based off of Strategic Compensation, Martocchio
A. How can you interface these two systems based on what you learned? Think about the limitations such as vacuum vs. pressure. 1. A gas chromatograph to a mass spectrometer 2. A liquid chromatograph...
A. How can you interface these two systems based on what you learned? Think about the limitations such as vacuum vs. pressure. 1. A gas chromatograph to a mass spectrometer 2. A liquid chromatograph to a mass spectrometer
A. How can you interface these two systems based on what you learned? Think about the limitations such as vacuum vs. pressure. 1. A gas chromatograph to a mass spectrometer 2. A liquid chromatograph to a mass spectrometer
Harwell, Inc. produces two different products, Product A and Product B. Harwell uses a traditional volume-based...
Harwell, Inc. produces two different products, Product A and Product B. Harwell uses a traditional volume-based costing system in which direct labor hours are the allocation base. Harwell is considering switching to an ABC system by splitting its manufacturing overhead cost of $850,000 across three activities: Setup, Production, and Finishing. Under the traditional volume-based costing system, the predetermined overhead rate is $8.50/direct labor hour. Under the ABC system, the rate for each activity and usage of the activity drivers are...
Carter, Inc. produces two different products, Product A and Product B. Carter uses a traditional volume-based...
Carter, Inc. produces two different products, Product A and Product B. Carter uses a traditional volume-based costing system in which direct labor hours are the allocation base. Carter is considering switching to an ABC system by splitting its manufacturing overhead cost of $783,000 across three activities: Design, Production, and Inspection. Under the traditional volume-based costing system, the predetermined overhead rate is $2.61/direct labor hour. Under the ABC system, the rate for each activity and usage of the activity drivers are...
Harwell, Inc. produces two different products, Product A and Product B. Harwell uses a traditional volume-based...
Harwell, Inc. produces two different products, Product A and Product B. Harwell uses a traditional volume-based costing system in which direct labor hours are the allocation base. Harwell is considering switching to an ABC system by splitting its manufacturing overhead cost of $750,000 across three activities: Setup, Production, and Finishing. Under the traditional volume-based costing system, the predetermined overhead rate is $7.50/direct labor hour. Under the ABC system, the rate for each activity and usage of the activity drivers are...
After a plasmid vector has been linearized using one restriction endonuclease, DNA Ligase cannot recircularize the plasmid unless it contains an insert DNA fragment to be cloned True or False True False
A. How can you interface these two systems based on what you learned? Think about the limitations such as vacuum vs. pressure. 1. A gas chromatograph to a mass spectrometer 2. A liquid chromatograph to a mass spectrometer
A. How can you interface these two systems based on what you learned? Think about the limitations such as vacuum vs. pressure. 1. A gas chromatograph to a mass spectrometer 2. A liquid chromatograph to a mass spectrometer
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