EXTRATION OF BANANA DNA USING SALT, DISTILLED WATER, DETERGENT, PROTEOLYTIC AGENT(ex: Protease or pineapple juice) AND 70-95% ISOPRORYL ALCOHOL.
1. What type of organisms and cells contain DNA?
2. What is the role of DNA in organisms?
3. Why would you want to extract the DNA from the organism?
4. In the procedure, why is the banana crushed or ground up into a pulp?
5. Why is detergent added to the pulp?
6. Look up the meaning of the term “bain-marie” (I have never heard of it either.) What is a bain-marie? Give a reference.
7. What is a good natural source of protease?
8. What does a protease do?
9. Compare the solubility of DNA in water and in alcohol.
10. What is the difference between a filtrate and a precipitate? If you don’t know, look it up. You need to know in order to perform the experiment properly.
11. Macroscopically, what did your DNA look like? Was it pink as in the lab write-up? Try to pull some out with a stick or a tweezers. What’s it like?
Thank you
Hi Answer:
Q.1.What type of organisms and cells contain DNA?
Answer: All most all prokaryotic and eukaryotic cells contain DNA as the genetic material. DNA is present in nucleus of the cells, In human body there are certain cells such as mature Red Blood cells and skin cells (cornified cells) don’t have nucleus so they also lack DNA
Q.2. What is the role of DNA in organisms?
Answer: Deoxyribonucleic acid (DNA) is act as genetic material of the organism and it contain all genetic information which are required for the development and proper functioning of the organism. It stores the information and passes them to one generation to another.
Q.3. Why would you want to extract the DNA from the organism?
Answer: DNA is within the nucleus of cell we want to extract the DNA for an organism to study the genetic makeup of the organism.
Q.4. In the procedure, why is the banana crushed or ground up into a pulp?
Answer: As we known that DNA is bounded in the nucleus and nucleus is present in side the cell so to take the DNA out from the cell we have to crush the banana cells so that DNA comes out from the cell.
Q.5. Why is detergent added to the pulp?
Answer: As detergent contain SDS/SLS (sodium laurel supfaye), this detergent is used to clean or removing the lipids and proteins present in the membrane of cells. When add detergent to the pulp it pull away the all lipids and proteins apart from and help in the release of DNA.
Q.6. Look up the meaning of the term “bain-marie” (I have never heard of it either.) What is a bain-marie? Give a reference.
Answer : A bain-marie is the type of heating instrument used in biology which is also known as water bath or double boiler. This instrument is used to heat the material gradually at a fixed temperature. It is also used to keep any material hot or warm for a particular time period.
Q.7. What is a good natural source of protease?
Answer: Protease are the enzymes which break down the proteins in to its monomer units i.e. amino acids. The natural source of proteases are the plants (44%) and microorganisms (18%). The major plant proteases are Papain, bromelain, keratinases. Whereas Bacillus sphaericus NRC 24,Mutant strain of Bacillus subtilis are the microbial strains which produces proteases.
Q.8. What does a protease do?
Answer: Protease are the enzymes which break down the proteins in to its monomer units i.e. amino acids
Q.9. Compare the solubility of DNA in water and in alcohol.
Answer: DNA is water soluble because it is a polar molecule and due to its polarity it is water soluble. Whereas DNA is non soluble in alcohol and it concentrate or precipitate the DNA.
Q.10. What is the difference between a filtrate and a precipitate? If you don’t know, look it up. You need to know in order to perform the experiment properly.
Answer: Filtrate is the liquid which remains after the centrifugation whereas the precipitates is the solid part which settles down in the tube after centrifugation.
Q.11. Macroscopically, what did your DNA look like? Was it pink as in the lab write-up? Try to pull some out with a stick or a tweezers. What’s it like?
Answer: The extracted DNA look like a white twisted thread which form the clumps having string like strands. DNA look white in color.
EXTRATION OF BANANA DNA USING SALT, DISTILLED WATER, DETERGENT, PROTEOLYTIC AGENT(ex: Protease or pineapple juice) AND...