Question

3.1 Osmotic Cell Disruption Use the van’t Hoff relationship to estimate the osmotic pressure drop across the membrane of a cell undergoing rupture in a 0.01 M salt solution, assuming the internal salt concentration is 0.2 M and that all salts are fully dissociated. Would you call this a negligible, ordinary, or large pressure drop? Why?

Answer 1

Π = iMRT

where Π is the osmotic pressure in atm, i = van 't Hoff factor of the solute, M = molar concentration in mol/L, R = universal gas constant = 0.08206 L·atm/mol·K, and T = absolute temperature in Kelvin.

Osmotic pressure is dircetly proportional to molar concentration. As in question molar concentration differs majorily that causes all salt dissociated means a large pressure drop.

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