The non-coding spacer regions (barcodes) differ from species to species due to insertions and deletions in the DNA sequence. We expect our products from the three primers sets will be approximately 600 bp long (+/- 100 bp). Given that our polymerase extends at 1 kb/minute, how long should we set our extension time for?
So expected products will be 600+/- 100 , so we will set extetion time for 700bo , and it should not be too long as than that , which created further problems.
Thus , extention time = 700/1K/min = 0.7 min × 60 sec = 42 sec.
So, extention time should be close to 42-45 sec.
The non-coding spacer regions (barcodes) differ from species to species due to insertions and deletions in...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...