You have isolated mouse C57BL6 trophoblast stem cells and have propagated them on MEFs with heparin....
You have isolated mouse C57BL6 trophoblast stem cells and have propagated them on MEFs with heparin. To test for the success of your culture protocol, you are injecting the cells into a mouse C57BL6 blastocyst. Once the pups are born, you notice that your experiment failed. Why? What was the expected outcome of this injection?
Homework Answers
1. Trophoblast is the layer that sourrounds the balstocyst becomes placenta that supplies blood and nutrient from mother to fetus. Poor placental formation results in low bloods nd nutrient supply and babies born out of this condition will be small and premature
If the experiment failed or culture failed to grow, placenta will not be formed properly and the pups will be smaller and premature due to less nutrient supply. If trophoblast stem cells injected has grown well, the baby pups will be healthy due to normal placental development
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