A relaxed B-type double stranded circular DNA molecule of 8,400 base pairs was cleaved by an E. coli Type II topoisomerase. What was the original linking number (Lko) and the final linking number (Lk) for this DNA molecule?
a. Lko=1,200; Lk=1,199
b. Lko=800; Lk=802
c. Lko-400; Lk=401
d. Lko+800; Lk=798
e. Lko=400; Lk=399
An explanation of the answer would be appreciated, thanks!
The rate of bp/turn = 10.5.
A relaxed B-type double stranded circular DNA molecule of 8,400 base pairs was cleaved by an...
This figure represents supercoiled, circular, double-stranded DNA: If this molecule of DNA originated as a linear molecule with a linking number (L) of 30, which was then circularized and unwound, the L for the unwound circular molecule would be O32 O 18 O 28 O 30 20