The susceptibility tests included in this unit are typically used differentiate between Gram-positive cocci. Would you...
Homework Answers
Rapid identification (ID) and antimicrobial susceptibility testing (AST) of the causative agent(s) of bloodstream infections (BSI) are essential for the timely selection of an appropriate antimicrobial therapy, which may result in a better outcome for patients, and contribute to contrast the emergence of antimicrobial resistance .
Gram negative organism possess the outer membrane which make them less susceptible for antibiotics and tests.
On the contraty, gram positive organism doesn't possess outer membrane and are more susceptible to tests and antibiotics.
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The susceptibility tests included in this unit are typically
used differentiate between Gram-positive cocci. Would you...
5. You use the Gram stain to try to differentiate bacteria in a sample. You find...
5. You use the Gram stain to try to differentiate bacteria in a sample. You find that all of the cells are Gram negative, but the teaching assistant insists there are both Gram positive and Gram negative cells present in the sample you are given. You definitely smeared a loop of the sample onto the slide and your fingers are stained a little purple and also a little red from handling the sides of the slide. Give a reason why...
Microbiology - Gram Positive (ONLY answer) [Q1] How can you differentiate Staphylococcus and Streptococcus under the...
Microbiology - Gram Positive (ONLY answer) [Q1] How can you differentiate Staphylococcus and Streptococcus under the microscope? [Q2] Your organism is catalase negative and produces a black precipiate on bile. What other test would likely be positive?
12. Which of the following reagents or tests would you use to differentiate between the following...
12. Which of the following reagents or tests would you use to differentiate between the following two compounds? Select only one answer and explain why you selected that answer.) C-H a) Chromic acid b) Benedict's test c) lodoform 13. Which of the following reagents or tests would you use to differentiate between the following two compounds? Sclect only one answer and explain why you selected that answer.) -H a) Chromic acid b) Benedict's test c) lodoform 14. An unknown compound...
You are given a mixed culture of two organisms in a small tube of TSB.
You are given a mixed culture of two organisms in a small tube of TSB. These two organisms include a Gram-negative strain (your bacterial strain to identify) and a Gram-positive strain (a contaminant). Your first task is to isolate these organisms which will help you identify which Gram-negative strain you are working with. 1) You inoculate two streak plates on TSA media and MacConkey Agar using aseptic technique. For each medium, state whether the medium is selective, differential, or neither and...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
66a. If you were to design a pathogenic bacteria justify why your bacteria would be Gram-positive...
66a. If you were to design a pathogenic bacteria justify why your bacteria would be Gram-positive or Gram- negative.
The chart below describes the biochemical reactions of six different Gram-positive bacteria for the indicated tests....
The chart below describes the biochemical reactions of six
different Gram-positive bacteria for the indicated tests. Following
the example your instructor presented at the beginning of the lab
period, construct a dichotomous key to identify the six bacteria
listed below. IMPORTANT: do not start your key with the same test
your instructor used to start the example in class. Draw your chart
by hand on the next page titled “Dichotomous Key.” Your key should
include all the tests needed to...
what results would you get when testing these organisms (Klebsiella pneumoniae, Escherichia coli, Staphylococcus saprophyticus, Proteus...
what results would you get when testing these organisms (Klebsiella pneumoniae, Escherichia coli, Staphylococcus saprophyticus, Proteus mirabilis, Proteus vulgaris, Providencia stuarti, Pseudomonas aeruginosa, Enterobacter aerogenes, Morganella morganii) Tests being run: MacConkey agar test, a Gram staining test, SIM agar and Urea agar (just indicate if results are negative or positive)
There may be more Which of the bacteria (A-E) would you expect to be catalase positive...
There may be more Which of the bacteria (A-E) would you expect to be catalase positive and why? than one of the bacteria positive for catalase. A OBLIGATE AEROBE B FACULTATIVE ANAEROBE CAEROTOLERANT ANAEROBE D OBLIGATE ANAEROBEE MICROAEROPHILE Extra: In a Gram stain one step could be omitted and still allow differentiation between Gram positive and Gram negative bacteria. What is that step? Why?
Laboratory & Exercises 1. You will not be physically performing these activities. However, you will be...
Laboratory & Exercises 1. You will not be physically performing these activities. However, you will be responsible for knowing how to perform and interpret them. View the following video clips: • SE Broth & Bile Esculin • CAMP Test 2. Review how to interpret the following tests by completing the chart: Positive Result Negative Result Gram Stain for cocci and bacilli purple Schaeffer-Fulton Stain for Endospores Negative Stain for Capsules Catalase (Reagent: Hydrogen peroxide) Coagulase (Reagent: Rabbit Plasma) Oxidase (Reagent:...
5. You use the Gram stain to try to differentiate bacteria in a sample. You find that all of the cells are Gram negative, but the teaching assistant insists there are both Gram positive and Gram negative cells present in the sample you are given. You definitely smeared a loop of the sample onto the slide and your fingers are stained a little purple and also a little red from handling the sides of the slide. Give a reason why...
12. Which of the following reagents or tests would you use to differentiate between the following two compounds? Select only one answer and explain why you selected that answer.) C-H a) Chromic acid b) Benedict's test c) lodoform 13. Which of the following reagents or tests would you use to differentiate between the following two compounds? Sclect only one answer and explain why you selected that answer.) -H a) Chromic acid b) Benedict's test c) lodoform 14. An unknown compound...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
66a. If you were to design a pathogenic bacteria justify why your bacteria would be Gram-positive or Gram- negative.
The chart below describes the biochemical reactions of six
different Gram-positive bacteria for the indicated tests. Following
the example your instructor presented at the beginning of the lab
period, construct a dichotomous key to identify the six bacteria
listed below. IMPORTANT: do not start your key with the same test
your instructor used to start the example in class. Draw your chart
by hand on the next page titled “Dichotomous Key.” Your key should
include all the tests needed to...
There may be more Which of the bacteria (A-E) would you expect to be catalase positive and why? than one of the bacteria positive for catalase. A OBLIGATE AEROBE B FACULTATIVE ANAEROBE CAEROTOLERANT ANAEROBE D OBLIGATE ANAEROBEE MICROAEROPHILE Extra: In a Gram stain one step could be omitted and still allow differentiation between Gram positive and Gram negative bacteria. What is that step? Why?
Laboratory & Exercises 1. You will not be physically performing these activities. However, you will be responsible for knowing how to perform and interpret them. View the following video clips: • SE Broth & Bile Esculin • CAMP Test 2. Review how to interpret the following tests by completing the chart: Positive Result Negative Result Gram Stain for cocci and bacilli purple Schaeffer-Fulton Stain for Endospores Negative Stain for Capsules Catalase (Reagent: Hydrogen peroxide) Coagulase (Reagent: Rabbit Plasma) Oxidase (Reagent:...
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