When the regulatory region of the VND6 gene is fused to a GFP marker gene and...
When the regulatory region of the VND6 gene is fused to a GFP marker gene and a terminator and the construct introduced into plants, GFP is expressed in immature vascular cells. When the transcription unit of the VND6 gene is fused to a regulatory region that provides expression in non-vascular mesophyll cells in transgenic plants (i.e. misexpression), these cells turn into vessel elements that are normally found only in vascular tissues and are used for water transport. Based on these experiments, what is the function of the VND6, a. regulatory region? b. transcription unit? c. gene?
Homework Answers
Individual functions of VND6
regulatory region, transcription unit and gene , as inferred from
the question.
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When the regulatory region of the VND6 gene is fused to a GFP
marker gene and...
You are studying the regulatory DNA of a mouse gene expressed in developing heart, liver, and...
You are studying the regulatory DNA of a mouse gene expressed in developing heart, liver, and lung tissue. Your preliminary work has shown that heart and lung expression of this gene is controlled by a short fragment of DNA just upstream of the promoter. Based on this result, you decide to investigate this region further to understand its function. You decide to compare this sequence to the regulatory DNA of the same gene found in rats and humans. Using genome...
You are conducting an experiment identifying enhancers that regulate the expression of a gene that codifies...
You are conducting an experiment identifying enhancers that
regulate the expression of a gene that codifies for a protein that
participates in cell division. The gene is called Mitosis
Regulatory Protein A or MRPA. The complete DNA sequences
for the MRPA promoter and coding region have been
identified. However, it is unknown if MRPA has enhancers
regulating its transcription. To answer this question your lab
first produced a transgenic cell line where GFP has been inserted
as a reporter gene....
molecular biology Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory r...
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the DNA immediately upstream of the transcription start site, but expression of the gene can also be affected by control elements. These can be thousands of base pairs upstream of the promoter, grouped in an enhancer. Because the distance and spacing of these control elements make them difficult to identify, scientists begin by deleting sections of DNA that contain possible control elements and measuring the...
PLEASE ANSWER ALL THE QUESTIONS: 1.What is true of tRNA (transfer RNA)? A they contain an...
PLEASE ANSWER ALL THE QUESTIONS: 1.What is true of tRNA (transfer RNA)? A they contain an anti-codon B they carry an amino acid C they can interpret the genetic code D all of these are true 2. How can transcription factors bound to distant enhancers influence gene expression? A the transcription factors can slide along the DNA until they get to the gene's promoter B DNA can loop, bringing these proteins into contact with the gene's promoter C both of...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the Unit gone and inserted in rat zygotes (by microinjection) a recombinant construct containing the Uni-1 coding sequence its entire 5 UTR region, covering both the minimal promoter and a series of cutative regulatory als elements. The mutantrats develop glorious, luminous hair. Hagrid has also discovered that the UNI-1 protein binds very tightly to DNA and hypothesizes it is a transcription factor that regulates proliferation...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
You are studying the regulatory DNA of a mouse gene expressed in developing heart, liver, and lung tissue. Your preliminary work has shown that heart and lung expression of this gene is controlled by a short fragment of DNA just upstream of the promoter. Based on this result, you decide to investigate this region further to understand its function. You decide to compare this sequence to the regulatory DNA of the same gene found in rats and humans. Using genome...
You are conducting an experiment identifying enhancers that
regulate the expression of a gene that codifies for a protein that
participates in cell division. The gene is called Mitosis
Regulatory Protein A or MRPA. The complete DNA sequences
for the MRPA promoter and coding region have been
identified. However, it is unknown if MRPA has enhancers
regulating its transcription. To answer this question your lab
first produced a transgenic cell line where GFP has been inserted
as a reporter gene....
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the Unit gone and inserted in rat zygotes (by microinjection) a recombinant construct containing the Uni-1 coding sequence its entire 5 UTR region, covering both the minimal promoter and a series of cutative regulatory als elements. The mutantrats develop glorious, luminous hair. Hagrid has also discovered that the UNI-1 protein binds very tightly to DNA and hypothesizes it is a transcription factor that regulates proliferation...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
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