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1. The Hill Equation a) Derive the Hill Equation for a protein binding a single ligand (i.e. the ...
. For a protein P binding to a ligand L, derive the Scatchard equation that relates...
. For a protein P binding to a ligand L, derive the Scatchard equation that relates the inverse of the concentration of the bound form (1/[P:L]) to the inverse of the concentration of free form (1/[P]).
Protein A Protein B [PL), UM 0.00 0 0.000 0 1) (10 marks) You study ligand...
Protein A Protein B [PL), UM 0.00 0 0.000 0 1) (10 marks) You study ligand binding to two proteins, A and B. You measured the concentration, (PL), of the ligand-bound form of the protein at various ligand concentrations, [L]. The data are summarized in the two tables below. Note that you do not know the total concentration of the protein, but you know that adding more ligand did not noticeably change the bound- protein concentration. [L), UM [PL], uM...
Short answer questions: 21. Protein A has a binding site for ligand L with a Ka...
Short answer questions: 21. Protein A has a binding site for ligand L with a Ka of 10-6 M. Protein B has a binding site for ligand L with a Ka of 10'M. (a). Which protein has a higher affinity for ligand L? Explain your reasoning. (b). At what concentration of ligand L is proteins A half-saturated. At what concentration of L is protein B half-saturated. [L] y = [L] + Ka 22. A protein binds to a ligand L...
A member of my research group recently performed protein-ligand binding experiments. She used UV-Vis to determine...
A member of my research group recently performed protein-ligand binding experiments. She used UV-Vis to determine θ by monitoring the PLL The data she collected is provided below: 6. L mM 0.1 .0060 0.2 .0124 0.3 .0190 0.4 .0245 0.5 .0307 0.6 .0380 0.7 .0430 0.8 0481 0.9 0530 1.0 0591 40.0865 2.0 3.0 .170 4.0 227 5.0 283 7.5 10.0 .500 15.0 .640 20.0 721 30.0 .812 50.0 60.0 70.0 80.0 90.0 100 150 200 .889 .905 .917 0.927...
41. Which of the following statements about protein-ligand binding is correct? A) The K is equal...
41. Which of the following statements about protein-ligand binding is correct? A) The K is equal to the concentration of ligand when all of the binding sites are occupied. B) The K is independent of such conditions as salt concentration and pH. C) The larger the K. (association constant), the weaker the affinity. D) The larger the K. the faster is the binding. E) The larger the K, the smaller the K. (dissociation constant) 42. The ability of O, to...
1. (14pts) A. What are the functions of the signal recognition particle (SRP) and translocon in p...
PLEASE ANSWER ALL THE QUESTIONS IN EACH PART. THANK YOU!
1. (14pts) A. What are the functions of the signal recognition particle (SRP) and translocon in protein transport to the ER? SRP Translocon B. How does the translocon determine which orientation to insert membrane proteins? C. How do budding & fusion affect the orientation of integral membrane proteins during vesicle transport? D. Consider a ligand-gated ion channel at the plasma membrane that binds to ligands in the cytoplasm. From a...
biochemistry if you could please help me answer the following questions! EFT i 11) (6 pts)...
biochemistry
if you could please help me answer the following questions!
EFT i 11) (6 pts) Which types of symmetry are possible for a protein with six (6) identical subunits? (Select all correct answers) a) cyclic C b) cyclic C3 c) dihedral D2 d) dihedral D e) octahedral o f) icosahedral ро, Yo₂ - pO₂+ Pso 12) (6 pts) What is the fractional saturation of oxygen binding to myoglobin when the partial pressure of oxygen is 1.5 torr and dissociation...
The PFK1 enzyme is regulated by a number of factors in the cell, including the level of ATP. PFK1 is a homo- tetramer (four identical subunits) and ATP binds to multiple sites. The binding of ATP can...
The PFK1 enzyme is regulated by a number of factors in the cell, including the level of ATP. PFK1 is a homo- tetramer (four identical subunits) and ATP binds to multiple sites. The binding of ATP can be measured using radio-labelled ATP. The data presented below represents the triplicate determination of the binding of ATP to PFK in the absence and presence of 300 HM citrate Moles of bound ATP per mole enzyme -citrate +citrate [ATP] (uMTrial 1Trial 2 Trial...
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your work...
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: AG 30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate pyruvateP Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 HM, 1.85 mM and 140...
Protein A Protein B [PL), UM 0.00 0 0.000 0 1) (10 marks) You study ligand binding to two proteins, A and B. You measured the concentration, (PL), of the ligand-bound form of the protein at various ligand concentrations, [L]. The data are summarized in the two tables below. Note that you do not know the total concentration of the protein, but you know that adding more ligand did not noticeably change the bound- protein concentration. [L), UM [PL], uM...
Short answer questions: 21. Protein A has a binding site for ligand L with a Ka of 10-6 M. Protein B has a binding site for ligand L with a Ka of 10'M. (a). Which protein has a higher affinity for ligand L? Explain your reasoning. (b). At what concentration of ligand L is proteins A half-saturated. At what concentration of L is protein B half-saturated. [L] y = [L] + Ka 22. A protein binds to a ligand L...
A member of my research group recently performed protein-ligand binding experiments. She used UV-Vis to determine θ by monitoring the PLL The data she collected is provided below: 6. L mM 0.1 .0060 0.2 .0124 0.3 .0190 0.4 .0245 0.5 .0307 0.6 .0380 0.7 .0430 0.8 0481 0.9 0530 1.0 0591 40.0865 2.0 3.0 .170 4.0 227 5.0 283 7.5 10.0 .500 15.0 .640 20.0 721 30.0 .812 50.0 60.0 70.0 80.0 90.0 100 150 200 .889 .905 .917 0.927...
41. Which of the following statements about protein-ligand binding is correct? A) The K is equal to the concentration of ligand when all of the binding sites are occupied. B) The K is independent of such conditions as salt concentration and pH. C) The larger the K. (association constant), the weaker the affinity. D) The larger the K. the faster is the binding. E) The larger the K, the smaller the K. (dissociation constant) 42. The ability of O, to...
PLEASE ANSWER ALL THE QUESTIONS IN EACH PART. THANK YOU!
1. (14pts) A. What are the functions of the signal recognition particle (SRP) and translocon in protein transport to the ER? SRP Translocon B. How does the translocon determine which orientation to insert membrane proteins? C. How do budding & fusion affect the orientation of integral membrane proteins during vesicle transport? D. Consider a ligand-gated ion channel at the plasma membrane that binds to ligands in the cytoplasm. From a...
biochemistry
if you could please help me answer the following questions!
EFT i 11) (6 pts) Which types of symmetry are possible for a protein with six (6) identical subunits? (Select all correct answers) a) cyclic C b) cyclic C3 c) dihedral D2 d) dihedral D e) octahedral o f) icosahedral ро, Yo₂ - pO₂+ Pso 12) (6 pts) What is the fractional saturation of oxygen binding to myoglobin when the partial pressure of oxygen is 1.5 torr and dissociation...
The PFK1 enzyme is regulated by a number of factors in the cell, including the level of ATP. PFK1 is a homo- tetramer (four identical subunits) and ATP binds to multiple sites. The binding of ATP can be measured using radio-labelled ATP. The data presented below represents the triplicate determination of the binding of ATP to PFK in the absence and presence of 300 HM citrate Moles of bound ATP per mole enzyme -citrate +citrate [ATP] (uMTrial 1Trial 2 Trial...
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: AG 30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate pyruvateP Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 HM, 1.85 mM and 140...
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