Question

Usually, your partner subcultures on Mondays, and you feed on Wednesday. However, when you got to lab on Wednesday this week, it was obvious that your partner didn’t subculture. At this point, your cells are over 100% confluent, and you need to subculture them immediately, or they’re all going to transform or worse yet, die. Unfortunately, you only have 15 minutes of hood time available today. Write out a alternate protocol that will accomplish the main goals of a subculture, but excludes the centrifugation step such that it can be completed in the time you have. Your answer should have not only the steps of your modified procedure, but also should explain what steps they mimic in your old procedure, and why the new steps will work. Be sure to address A-D:

1. Identify a specific underlying problem. Your problem should NOT just be a restatement of the

   question!!

2. Show/explain why it’s a problem (including any math)

3. Give a solution(s) that will solve the specific problem you identified (including any math)

4. Explain how the solution(s) will solve the problem(s)

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Answer 1

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