Homework Answers
1. The colored pigment found in siling labuyo is RED , while in
malunggay leaves it is GREEN. The pigments of siling labuyo leaves
are carotene, zeaxanthin, lutein and -cryptoxanthin, and
anthocyanin. The pigments of malunggay leaves are Xanthophylls,
Chlorophyll A, and Chlorophyll B. The structures are given
below.
2. Among them carotene is least polar (hydrocarbon, no polar group) and Chlorophyll B will be most polar because of the presence of pyrrole ring, -CHO and -CO2Me group.
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EXPERIMENT 5 Chromatography 1. What are the colored pigments that can be found in red siling labn...
Pre lab Introduction Your instructor has prepared an extract of chloroplast pigments from fresh green grass...
Pre lab
Introduction Your instructor has prepared an extract of chloroplast pigments from fresh green grass or fresh spinach. A blender was used to rupture the cells, and the pigments were then extracted with acetone, an organic solvent. Work- ing with one other student, begin this exercise by separating the pigments extracted using paper chromatography. To do this, you will apply the pig- ment extract to a cylinder of chromatographic paper. You will then place the ylinder in a jar...
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is...
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
-Give a brief background on the role of pigments in photosythensis. -state your hypothesis. -finish with...
-Give a brief background on the role of pigments in
photosythensis.
-state your hypothesis.
-finish with stating the rationale for your hypothesis (how
did you arrive at these hypothesis?)
this is an introduction piece, so it doesn't need the results to
write the papee. introduction sections set up the background,
purpose, rationale, and hypothesis for a study.
Biod 20 PHOTOSYNTHESIS Background Plants harness the energy of the sun through a process called photosynthesis. The leaf is the plant organ that...
Certain pigments found in cells take on different colors depending upon the pH of their environment....
Certain pigments found in cells take on different colors depending upon the pH of their environment. For example, betacyanin (responsible for the red color in beets) and a variety of anthocyanins (responsible for the colors in many flowers) appear colored only under acidic conditions. In neutral or basic conditions, they are colorless. These pigments are usually found concentrated within the vacuoles of cells, where they are colored. From this, you can rightly surmise that the interior of a vacuole is...
E 3 Normal No spacing Heading 1 Heading 1. Plants sometimes store carbohydrates in their roots...
E 3 Normal No spacing Heading 1 Heading 1. Plants sometimes store carbohydrates in their roots or leaves in the form of which you tested for with iodine solution. 2. Which color of light (red, green or blue) resulted in the least amount ofphotosynthesis in th Geranium leaves? 3. What are the 2 products of photosynthesis? and 4. TRUE or FALSE: Plant cells get their ATP from photosynthesis and do not use cellular respiration. 5 TRUE or FALSE: Polar molecules...
1. Why is Para Red (as well as other dyes) colored? 2. Consider the amounts of...
1. Why is Para Red (as well as other dyes) colored? 2. Consider the amounts of reactants used in the lab to determine the limiting reagent and theoretical yield of Para Red dye. Show calculations. (If these calculations were detailed in the intro, just direct the grader to the page where they can be found). 3. Why do you have to keep the temperature < 50C when preparing the diazonium salt intermediate? 4. What process is used to dye blue...
Chromatography Basics Multiple Choice: Answer the following multiple choice questions about Chromatography Basics (SHOW WORKING): 1) In...
Chromatography Basics Multiple Choice: Answer the following multiple choice questions about Chromatography Basics (SHOW WORKING): 1) In a given chromatographic column, A) different solutes behave as if there are a different number of theoretical plates for each. B) the number of theoretical plates is the same for all solutes. C) efficiency is improved by increasing plate height. 2) What useful information can be found from a Van Deemter plot? A) The selectivity factor B) Optimum mobile phase flow rate C) Optimum...
Lab Day 8: Chromatography Prelab Questions 1) The basic food colors you can buy are red,...
Lab Day 8: Chromatography Prelab Questions 1) The basic food colors you can buy are red, blue areen, and yellow. Think about what you know about colors and predict which of these might be a single colored compound and which ones might contain more than one. write down your prediction. If you have the colors listed and want a purple color, what would you do? 2) Put the following chromatography solvents in order of polarity as a chromatography solvent: acetone,...
6a) During experiment 7, you performed column chromatography in order to separate two main compounds from...
6a) During experiment 7, you performed column chromatography in order to separate two main compounds from tomato paste. What are these two compounds? 6b) Is this separation technique a chemical or physical method of separation? 6c) What color is each of these compounds? What about their molecular structure gives rise to their color? 6d) During the experiment, we only observed one band on the column even though we should have observed two. However, you may have noticed, we had in...
Draw the zwitterionic structures of the five amino acids to be examined in this experiment. What...
Draw the zwitterionic structures of the five amino acids to be examined in this experiment. What factors are going to determine how far a particular amino acid travels up the chromatography paper? Predict which class of amino acids (non-polar, neutral polar, addle, or basic) will move furthest and which will move the least.
Pre lab
Introduction Your instructor has prepared an extract of chloroplast pigments from fresh green grass or fresh spinach. A blender was used to rupture the cells, and the pigments were then extracted with acetone, an organic solvent. Work- ing with one other student, begin this exercise by separating the pigments extracted using paper chromatography. To do this, you will apply the pig- ment extract to a cylinder of chromatographic paper. You will then place the ylinder in a jar...
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
-Give a brief background on the role of pigments in
photosythensis.
-state your hypothesis.
-finish with stating the rationale for your hypothesis (how
did you arrive at these hypothesis?)
this is an introduction piece, so it doesn't need the results to
write the papee. introduction sections set up the background,
purpose, rationale, and hypothesis for a study.
Biod 20 PHOTOSYNTHESIS Background Plants harness the energy of the sun through a process called photosynthesis. The leaf is the plant organ that...
E 3 Normal No spacing Heading 1 Heading 1. Plants sometimes store carbohydrates in their roots or leaves in the form of which you tested for with iodine solution. 2. Which color of light (red, green or blue) resulted in the least amount ofphotosynthesis in th Geranium leaves? 3. What are the 2 products of photosynthesis? and 4. TRUE or FALSE: Plant cells get their ATP from photosynthesis and do not use cellular respiration. 5 TRUE or FALSE: Polar molecules...
Lab Day 8: Chromatography Prelab Questions 1) The basic food colors you can buy are red, blue areen, and yellow. Think about what you know about colors and predict which of these might be a single colored compound and which ones might contain more than one. write down your prediction. If you have the colors listed and want a purple color, what would you do? 2) Put the following chromatography solvents in order of polarity as a chromatography solvent: acetone,...
6a) During experiment 7, you performed column chromatography in order to separate two main compounds from tomato paste. What are these two compounds? 6b) Is this separation technique a chemical or physical method of separation? 6c) What color is each of these compounds? What about their molecular structure gives rise to their color? 6d) During the experiment, we only observed one band on the column even though we should have observed two. However, you may have noticed, we had in...
Draw the zwitterionic structures of the five amino acids to be examined in this experiment. What factors are going to determine how far a particular amino acid travels up the chromatography paper? Predict which class of amino acids (non-polar, neutral polar, addle, or basic) will move furthest and which will move the least.
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