Question

onate #2 6 8 26. The effect of an inhibitor (II) on the rate of a single-su investigated and gave the following results (three experiments at three different inhibitor concentrations [I]-0.0, 0.20 and 0.40 mmol/L): bstrate enzyme-catalyzed reaction was Substrate concentration [S] Inhibitor concentration [I] 0.0 mmol/L mmol / L) Rate of reaction (vo) (umol /L/s) 0.05 0.10 0.20 0.40 0.60 0.033 0.055 0.083 0.126 Substrate concentration [S (mmol / L) Inhibitor concentration [I] 0.20 mmol/L Rate of reaction (Vo) (umol/L/s) 0.05 0.10 0.20 0,40 0.60 0.026 0.045 0.071 0.100 0.116 Substrate concentration [S] Inhibitor concentration U] 0.40 mmol/L (mmol /L) Rate of reaction (ve) (umol/L/s) 0.05 0.10 0.20 0.40 0.60 0.021 0.038 0.062 0.091 0.108 Using the above data, answer the following questions (a) Is the inhibitor a mixed, uncompetitive or competitive inhibitor? (b) The maximum reaction velocity is (c) The value for K is_ (d) An approximate value for K, is

Answer 1

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max ma k shure LI] Tnhibihen fur

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fr Senne Vt diffeent Km the inhibition is competitive Inibitoys all 3 ernments is