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The calcium ion binds to a certain protein to form a 1:1 complex. When 96μM protein and 60μM Ca2+...

The calcium ion binds to a certain protein to form a 1:1 complex. When 96μM protein and 60μM Ca2+ are mixed together, the concentration of free ligand (calcium) was found to be 31μM.

A) Calculate the dissociation constant of the Ca2+ −protein complex.

B) What concentration of Ca2+ would you need to dissolve in the solution to ensure that 95% of the protein is bound to calcium?

C) What percentage of the calcium ions are free in the conditions of part B?

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The calcium ion binds to a certain protein to form a 1:1 complex. When 96μM protein and 60μM Ca2+ are mixed together, the concentration of free ligand (calcium) was  found to be  31μM.

Solutions 2+ lyboM a) Egur/1bium 31.HM 6 ,ΗΜ Change = -C60-31),UM 31x64 136 02 The he chss ociated Constant eļ the 02-Robin Comp 31x67 e9 = |チ1 : 62 96UM k 2 C-1 2) ニ 1.396x62OY GI-2 8 X 1.39시 - 136 (or) 452 23 1452-23 uMa2teed to bedrssoved 1 X 100 45 2-23-91-2 xloo 45 2.23 19342 %

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