Lane 1 has Eco R1 and Sa2 digestion. There is only two restriction site that's why two bands are produced.
Lane 2 has hind3 and sa2 digestion. There is also two bands produced.
Lane 3 has ECoR5 , Sa2 and Eco R1 digestion. There are three restriction sites. Hence 3 bands produced.
I have taken restriction site base from vector structure.
Pvull CAG CTG 6. List three key characteristics of an expression vector 7. Gel electrophoresis ca...
Looking at the gel electrophoresis of pcDNA vector digestion with no enzyme, Xbal, or EcoRI below, answer the following question (#76). Remember pcDNA vector has both Xbal and EcoRI restriction enzyme sites within the multiple cloning sequence. GELA no enz Xbal Eceri ladder GEL B no enz_Xbal EceRl ladder 76. Which gel showed successful digestion (cutting) of the vector? A. Gel A B. Gel B C. Both A and B D. Neither Gel A or B E. Gel electrophoresis is...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel electrophoresis, the DNA fragments can be separated on a gel, based on their lengths. In order to see the fragments, a stain is typically added to the gel. The size of each fragment can be determined by comparing each one to a DNA molecular weight marker of known size. Below is a map of pBR22 plasmid. The position and base pair number of the...